LOCUS       sequence-69486-e        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 
            termination efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB1902
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(105..199)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(230..1024)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(341..360)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     951..970
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cttattaatc agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca
       61 cctgaagtca gccccatacg atataagttg ttactagtgc ttggattctc accaataaaa
      121 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
      181 ggatctatca acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact
      241 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
      301 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
      361 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
      421 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
      481 cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
      541 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
      601 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
      661 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
      721 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
      781 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
      841 cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
      901 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
      961 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     1021 tcatgcgaaa cgatcct
//
