LOCUS       sequence-69490-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring trp_att_L126 termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB801
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      41..68
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(139..156)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(353..372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
       61 tttctgcgtt tataggatcc taactcgagc tgaagccaat agggataaca gggtaatcct
      121 agggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
      181 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
      241 cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
      301 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
      361 caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
      421 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
      481 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
      541 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact
      601 ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag
      661 gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca
      721 aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt ttt
//
