LOCUS       sequence-69492-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring T7 early termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB803
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      42..69
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(140..157)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(354..373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
       61 ctttctgcgt ttataggatc ctaactcgag ctgaagccaa tagggataac agggtaatcc
      121 tagggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
      181 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
      241 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
      301 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
      361 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
      421 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
      481 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct
      541 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac
      601 tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa
      661 ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc
      721 aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt ttt
//
