LOCUS       sequence-69515-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Sun J, Lau A, Wang X, Liao TY, Zoubeidi A, Hmama Z
  TITLE     A broad-range of recombination cloning vectors in mycobacteria.
  JOURNAL   Plasmid. 2009 Nov . 62(3):158-65.
  PUBMED    19647016
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid. 
            2009 Nov . 62(3):158-65."
COMMENT     Sequence Label: pJAK1.B
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(132..256)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      325..371
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(521..540)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(605..624)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      181 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
      241 tcttgtacaa agtggttgat ctgcagaatt cgaagcttat cgatgtcgac gtagttaact
      301 agcgtacgat cgactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      361 tttcgtttta tgccatcatg gccgcggtga tcagctagcc acctgacgtc gggggggggg
      421 gaaagccacg ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
      481 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
      541 attcaacggg aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga tgctgattta
      601 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
      661 tatgggaagc cccatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
      721 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
      781 atcaagcatt ttatccgtac tcctga
//
