LOCUS       sequence-69523-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Sun J, Lau A, Wang X, Liao TY, Zoubeidi A, Hmama Z
  TITLE     A broad-range of recombination cloning vectors in mycobacteria.
  JOURNAL   Plasmid. 2009 Nov . 62(3):158-65.
  PUBMED    19647016
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid. 
            2009 Nov . 62(3):158-65."
COMMENT     Sequence Label: pJAK2.D
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(121..245)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      314..360
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(510..529)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(594..613)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
       61 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
      121 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
      181 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
      241 gtggttgatc tgcagaattc gaagcttatc gatgtcgacg tagttaacta gcgtacgatc
      301 gactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      361 gccatcatgg ccgcggtgat cagctagcca cctgacgtcg gggggggggg aaagccacgt
      421 tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
      481 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
      541 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
      601 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc
      661 ccatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
      721 tgagatgg
//
