LOCUS       sequence-69536-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled 
            Inducible Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Bryan EM, Bae T, Kleerebezem M, Dunny GM
  TITLE     Improved vectors for nisin-controlled expression in gram-positive 
            bacteria.
  JOURNAL   Plasmid. 2000 Sep;44(2):183-90.
  PUBMED    10964628
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2000-09"; volume: "44(2)"; pages: "183-90"
COMMENT     Sequence Label: pMSP3535VA
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttggaaagt tgttcaagaa aatcaaggcg gagttagctc ttgatgatgt cgaagaaggg
       61 aatcttgttc agaccggagc agaagaatct gcagaaagta ctggtcgtga aattgttgcc
      121 ttttggaatt gggatagaaa gaattatttt gtgaggtagc tgatgagtaa aacagtatca
      181 gaattagctc aagaattggg agttagtagg cagtatctta atcggatttt atcgcaaaat
      241 aatctcggtc gaaaaaaagg gaataaaaaa gtagtttccg atatggacga gaaagtactc
      301 atatcaatac ttgagggcaa tattggaaac caaaaaacgg aaactaaaac ggaaacaaaa
      361 acattagttt ccaatatgtc tttaagctca agtgagataa gggaagaaga ccatattgga
      421 aatcgaaaaa tggaaaccaa attggaaact gaattagaaa ctagtttccg atatgtccgt
      481 gaacagttga aaataaagga aaaagaggtt gaacaacccc cggctttagg tatagtgtgt
      541 atctcaatcc ttggtatatt gaaaagaaag actaaaaatt gatagattat atttcttcag
      601 aatgaatggt ataatgaagt aatgagtact aaacaatcgg aggtaaagtg gtgtataaaa
      661 ttttaatagt tgatgatgat caggaaattt taaaattaat gaagacagca ttagaaatga
      721 gaaactatga agttgcgacg catcaaaaca tttcacttcc cttggatatt actgattttc
      781 agggatttga tttgattttg ttagatatca tgatgtcaaa tattgaaggg acagaaattt
      841 gtaaaaggat tcgcagagaa atatcaactc caattatctt tgttagtgcg aaagatacag
      901 aagaggatat tataaacggc ttaggtattg gtggggatga ctatattact aagcctttta
      961 gccttaaaca gttggttgca aaagtggaag caatataa
//
