LOCUS       sequence-69538-e        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  allow pharmacologically induced silencing of electrical activity in 
            CNS neurons upon exposure to the anthelmintic drug ivermectin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Frazier SJ, Cohen BN, Lester HA
  TITLE     An Engineered GluCl Channel for Sensitive, Consistent Neuronal 
            Silencing by Ivermectin.
  JOURNAL   J Biol Chem. 2013 May 29.
  PUBMED    23720773
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2013 May 29."
COMMENT     Sequence Label: pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F ...
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      152..161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga
      121 tccactagtc cagtgtggtg gaattgccct tgccaccatg gccacgtgga tcgtgggcaa
      181 gctgatcatc gcttccctga tcctggggat ccaggcccag caggcccgca ccaagagcca
      241 ggacatcttc gaggacgaca acgacaacgg cactaccacc ctggagtccc tggctcgcct
      301 gacctccccc atccacatcc ccatcgagca gccccagacc tccgacagca agatcctggc
      361 ccacctgttc accagcggct acgacttccg cgtgcgcccc cccaccgaca acggcggccc
      421 cgtggtggtg agcgtgaaca tgctgctgcg caccatcagc aagatcgacg tggtgaacat
      481 ggagtactcc gcccagctga ccctgcgcga gagctggatc gacaagcgcc tgtcctacgg
      541 cgtcaagggc gacggccagc ccgacttcgt catcctgacc gtgggccacc agatctggat
      601 gcccgacacc ttcttcccca acgagaagca ggcttacaag cacaccatcg acaagcccaa
      661 cgtcctgatc cgcatccaca acgacggcac cgtcctgtac tccgtccgca tcagcctggt
      721 gctgagctgc cccatgtacc tgcagtacta cccgatggac gtgcagcagt gcagcatcga
      781 cctggcctcc tacgcctaca ccaccaagga catcgagtat ctgtggaagg agcacagccc
      841 cctgcagctg aaggtcggcc tgtcctccag cctgcccagc ttccagctga ccaacaccag
      901 caccacctac tgcaccagcg tcaccaacac cggcatctac agctgcctga ggaccaccat
      961 ccagctgaag cgcgagttct ccttctacct gctgcaactg tacatcccca gctgcatgct
     1021 cgtgatcgtg tcctggg
//
