LOCUS       sequence-69540-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  allow pharmacologically induced silencing of electrical activity in 
            CNS neurons upon exposure to the anthelmintic drug ivermectin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Frazier SJ, Cohen BN, Lester HA
  TITLE     An Engineered GluCl Channel for Sensitive, Consistent Neuronal 
            Silencing by Ivermectin.
  JOURNAL   J Biol Chem. 2013 May 29.
  PUBMED    23720773
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2013 May 29."
COMMENT     Sequence Label: pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F ...
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(34..75)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(join(301..1011,1012..1014,1015..1017))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(343..364)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     692..711
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     951..972
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aactcaatgg tgatggtgat gatgaccggt acgcgtagaa tcgagaccga ggagagggtt
       61 agggataggc ttaccttcga accgcgggcc ctctagactc gagcggccgc cactgtgctg
      121 gatatctgca gaattgccct tgctagctca gaacagaacg ttctgctggc cgaagcggct
      181 ccagtacagg atgttgaaca cgaagaacag cacggggaac agggcgcggc tgatcaggtc
      241 cacgcgcttg ctgatgtcgt tccactcggt cttggtctcc agcaggttca ggaaggaatt
      301 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      361 catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactggtagc tcaggtagtg
      421 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      481 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
      541 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      601 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      661 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      721 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      781 catgtggtcg gggtagcggg cgaagcactg caggccgtag ccgaaggtgg tcacgagggt
      841 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
      901 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
      961 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatgga
     1021 attcatctgc cggcggtt
//
