LOCUS       sequence-69541-e       12340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac expression gateway destination vector with 
            Insulator-flanked EF1a promoter gateway cassette and floxed Hygro 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 12340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC150-PBLHL-4xHS-EF1a-DEST
FEATURES             Location/Qualifiers
     source          1..12340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   299..333
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    667..700
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        850..1229
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1230..1433
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1384..1404
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1430..1454
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2618..3613
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    3649..3783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3686..3705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3740..3759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    3812..3845
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4487..5665
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4718..5656
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5613..5633
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5726..5850
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5875..5880,5881..5898,5899..5905)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5959..6618
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6025..6044)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6960..7265
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(7306..7430)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7483..7524
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(7562..7579)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7568..7792
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8612..8674
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8712..8731)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8713..8731)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8738..8755)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8738..8754)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8747..8769)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8892..9194
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             9543..10337
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9543..10337
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9597..9616)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10207..10226
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(10587..11378,11379..11447))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11210..11229
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11571..12159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12060..12079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12313..12330
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttttaacc
      301 ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc
      361 taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc
      421 gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga
      481 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
      541 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
      601 agatatcaac tagaatgcta gacctttcgt cttcaagaat tccgatcata ttcaataacc
      661 cttaatataa cttcgtataa tgtatgctat acgaagttat taggtctgaa gaggagttta
      721 cgtccagcca agcttaggat cccgggtacc gatggccggg cctcgaggag cttggcccat
      781 tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac
      841 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      901 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      961 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     1021 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     1081 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     1141 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1201 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     1261 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     1321 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     1381 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
     1441 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
     1501 gggaccgatc cagcctcccc ggctagccgc cgccaccatg aaaaagcctg aactcaccgc
     1561 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     1621 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     1681 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     1741 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     1801 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     1861 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     1921 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1981 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     2041 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     2101 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     2161 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     2221 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     2281 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     2341 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     2401 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     2461 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtcg cgtctgcgtt
     2521 cgaccaggct gcgcgttctc gcggccatag caaccgacgt acggcgttgc gccctcgccg
     2581 gcagcaagaa gccacggaag tccgcccgga gcagaaaatg cccacgctac tgcgggttta
     2641 tatagacggt ccccacggga tggggaaaac caccaccacg caactgctgg tggccctggg
     2701 ttcgcgcgac gatatcgtct acgtacccga gccgatgact tactggcggg tgctgggggc
     2761 ttccgagaca atcgcgaaca tctacaccac acaacaccgc ctcgaccagg gtgagatatc
     2821 ggccggggac gcggcggtgg taatgacaag cgcccagata acaatgggca tgccttatgc
     2881 cgtgaccgac gccgttctgg ctcctcatat cgggggggag gctgggagct cacatgcccc
     2941 gcccccggcc ctcaccctca tcttcgaccg ccatcccatc gccgccctcc tgtgctaccc
     3001 ggccgcgcgg taccttatgg gcagcatgac cccccaggcc gtgctggcgt tcgtggccct
     3061 catcccgccg accttgcccg gcaccaacat cgtgcttggg gcccttccgg aggacagaca
     3121 catcgaccgc ctggccaaac gccagcgccc cggcgagcgg ctggacctgg ctatgctggc
     3181 tgcgattcgc cgcgtttacg ggctacttgc caatacggtg cggtatctgc agtgcggcgg
     3241 gtcgtggcgg gaggactggg gacagctttc ggggacggcc gtgccgcccc agggtgccga
     3301 gccccagagc aacgcgggcc cacgacccca tatcggggac acgttattta ccctgtttcg
     3361 ggcccccgag ttgctggccc ccaacggcga cctgtataac gtgtttgcct gggccttgga
     3421 cgtcttggcc aaacgcctcc gttccatgca cgtctttatc ctggattacg accaatcgcc
     3481 cgccggctgc cgggacgccc tgctgcaact tacctccggg atggtccaga cccacgtcac
     3541 cacccccggc tccataccga cgatatgcga cctggcgcgc acgtttgccc gggagatggg
     3601 ggaggctaac tgaagcttcc cgggggtacc gaattcgtcg acagatctaa cttgtttatt
     3661 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     3781 atcatcaagc ttaggatccg gaacccttaa tataacttcg tataatgtat gctatacgaa
     3841 gttattaggt ccctcgaaga ggttcactgg cgcgttggat ccccgggtac cgagttggga
     3901 gctcacgggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
     3961 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc
     4021 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
     4081 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc
     4141 tttaggctga aagagagatt tagaatgaca gtctagtggg agctcacggg gacagccccc
     4201 ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg
     4261 cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg
     4321 acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct
     4381 ctttgagcct gcagacacct ggggggatac ggggaaaaag ctttaggctg aaagagagat
     4441 ttagaatgac agaactcgat ttcattgcag actggcgcgc ccgtgaggct ccggtgcccg
     4501 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     4561 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     4621 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     4681 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     4741 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     4801 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     4861 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     4921 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     4981 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     5041 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     5101 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     5161 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     5221 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     5281 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     5341 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     5401 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     5461 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     5521 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     5581 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     5641 ttttcttcca tttcaggtgt cgtgaagaag cttggtaccg agctcggatc cactagtcca
     5701 gtgtggtgga attctgcaga tatcaacaag tttgtacaaa aaagctgaac gagaaacgta
     5761 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     5821 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
     5881 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     5941 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     6001 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     6061 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     6121 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     6181 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     6241 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     6301 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     6361 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     6421 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     6481 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     6541 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     6601 tggcagggcg gggcgtaaag atctggatcc ggcttactaa aagccagata acagtatgcg
     6661 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     6721 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     6781 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     6841 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     6901 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     6961 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     7021 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     7081 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     7141 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     7201 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     7261 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     7321 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     7381 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt tgatatccag
     7441 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac
     7501 cctctcctcg gtctcgattc tacgcgtacc ggttaggggc ccgtttaaac ccgctgatca
     7561 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7621 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7681 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7741 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcggcgcgc
     7801 ctggatcccc gggtaccgag ttgggagctc acggggacag ccccccccca aagcccccag
     7861 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     7921 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     7981 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     8041 cacctggggg gatacgggga aaaagcttta ggctgaaaga gagatttaga atgacagtct
     8101 agtgggagct cacggggaca gccccccccc aaagccccca gggatgtaat tacgtccctc
     8161 ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg
     8221 catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg
     8281 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg
     8341 aaaaagcttt aggctgaaag agagatttag aatgacagaa ctcgatttca ttgcagactg
     8401 gccggccact agtacgcgcc ggctcgacat actagttaaa agttttgtta ctttatagaa
     8461 gaaattttga gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg
     8521 aatttattat tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata
     8581 aataaacctc gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt
     8641 taacgtacgt cacaatatga ttatctttct agggttaaaa gggcgaattc gcggccgcta
     8701 aattcaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     8761 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     8821 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8881 tatacgtacg gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     8941 tggatgtaca gagtgatatt attgacacgc cggggcgacg gatggtgatc cccctggcca
     9001 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     9061 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     9121 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     9181 tctggggaat ataaatgtca ggcatgagat tatcaaaaag gatcttcacc tagatccttt
     9241 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     9301 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     9361 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     9421 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc
     9481 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc
     9541 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     9601 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     9661 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     9721 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     9781 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     9841 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     9901 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     9961 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
    10021 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
    10081 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
    10141 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
    10201 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
    10261 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
    10321 ttgacgagtt cttctgaatt attaacgctt acaatttcct gatgcggtat tttctcctta
    10381 cgcatctgtg cggtatttca caccgcatca ggtggcactt ttcggggaaa tgtgcgcgga
    10441 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagattatca
    10501 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    10561 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    10621 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    10681 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10741 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10801 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10861 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10921 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10981 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    11041 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    11101 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    11161 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    11221 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    11281 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    11341 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    11401 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    11461 caatattatt gaagcattta tcagggttat tgtctcatga ccaaaatccc ttaacgtgag
    11521 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    11581 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    11641 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    11701 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    11761 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    11821 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    11881 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11941 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    12001 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    12061 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    12121 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    12181 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    12241 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    12301 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
