LOCUS       sequence-69548-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR
            array designed for the easy cloning of new spacers..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
  TITLE     Programmable repression and activation of bacterial gene expression 
            using an engineered CRISPR-Cas system.
  JOURNAL   Nucleic Acids Res. 2013 Jun 12.
  PUBMED    23761437
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Jun 12."
COMMENT     Sequence Label: pdCas9
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(87..92,93..109,110..115)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(433..511)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
       61 tacgatataa gttgtaattc taatgtttga cagcttatca tcgataagct ttaatgcggt
      121 agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
      181 ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
      241 gtactgccgg gcctcttgcg ggattacgaa atcatcctgt ggagcttagt aggtttagca
      301 agatggcagc gcctaaatgt agaatgataa aaggattaag agattaattt ccctaaaaat
      361 gataaaacaa gcgttttgaa agcgcttgtt tttttggttt gcagtcagag tagaatagaa
      421 gtatcaaaaa aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat
      481 tttaacttgc tatgctgttt tgaatggttc caacaagatt attttataac ttttataaca
      541 aataatcaag gagaaattca aagaaattta tcagccataa aacaatactt aatactatag
      601 aatgataaca aaataaacta ctttttaaaa gaattttgtg ttataatcta tttattatta
      661 agtattgggt aatatttttt gaagagatat tttgaaaaag aaaaattaaa gcatattaaa
      721 ctaatttcgg aggtcattaa aactattatt gaaatcatca aactcattat ggatttaatt
      781 taaacttttt attttaggag gcaaaaatgg ataagaaata c
//
