LOCUS       sequence-69549-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR
            array designed for the easy cloning of new spacers..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
  TITLE     Programmable repression and activation of bacterial gene expression 
            using an engineered CRISPR-Cas system.
  JOURNAL   Nucleic Acids Res. 2013 Jun 12.
  PUBMED    23761437
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Jun 12."
COMMENT     Sequence Label: pdCas9
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcagctttc gttaaattat caaacttacg ttgagtgatt aacttggcgt ttagaagttg
       61 tctccaatag tttttcatct ttttgactac ttcttcactt ggaacgttat ccgatttacc
      121 acgattttta tcagaacgcg ttaagacctt attgtctatt gaatcgtctt taaggaaact
      181 ttgtggaaca atggcatcga catcataatc acttaaacga ttaatatcta attcttggtc
      241 cacatacatg tctcttccat tttggagata atagagatag agcttttcat tttgcaattg
      301 agtattttca acaggatgct ctttaagaat ctgacttcct aattctttga taccttcttc
      361 gattcgtttc atacgctctc gcgaattttt ctggcccttt tgagttgtct gattttcacg
      421 tgccatttca ataacgatat tttctggctt atgccgcccc attactttga ccaattcatc
      481 aacaactttt acagtctgta aaataccttt tttaatagca gggctaccag ctaaatttgc
      541 aatatgttca tgtaaactat cgccttgtcc agacacttgt gctttttgaa tgtcttcttt
      601 aaatgtcaaa ctatcatcat ggatcagctg cataaaattg cgattggcaa aaccatctga
      661 tttcaaaaaa tctaatattg ttttgccaga ttgcttatcc ctaataccat taatcaattt
      721 tcgagacaaa cgtccccaac cagtataacg gcgacgttta agctgtttca tcaccttatc
      781 atcaaagagg tgagcatatg ttttaagtct ttcctcaatc at
//
