LOCUS       sequence-69552-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  TITLE     Strehler unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EGFP-hCaM
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..44
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..80
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             138..587
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPT
                     EAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAA
                     ELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     complement(765..784)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agtccggact cagatcctac ccctatgatg tgcctgacta tgcccaccac caccaccacc
       61 acctggtgcc caggggcagc agatctcgag ctcaagcttc gaattctgca gtcgacggta
      121 cccacaggaa acagaccatg gctgaccagc tgactgagga gcagattgca gagttcaagg
      181 aggccttctc cctctttgac aaggatggag atggcactat caccaccaag gagttgggga
      241 cagtgatgag atccctggga cagaacccca ctgaagcaga gctgcaggat atgatcaatg
      301 aggtggatgc agatgggaac gggaccattg acttcccgga gttcctgacc atgatggcca
      361 gaaagatgaa ggacacagac agtgaggagg agatccgaga ggcgttccgt gtctttgaca
      421 aggatgggaa tggctacatc agcgccgcag agctgcgtca cgtaatgacg aacctggggg
      481 agaagctgac cgatgaggag gtggatgaga tgatcaggga ggctgacatc gatggagatg
      541 gccaggtcaa ttatgaagag tttgtacaga tgatgactgc aaagtgaagg ccccccgggg
      601 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
      661 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
      721 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      781 tttcacaaat aaagcatttt tt
//
