LOCUS       sequence-69553-e        1965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genetically encoded fluorescent indicator for simultaneous PIP3 and 
            hydrogen peroxide detection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Mishina NM, Bogeski I, Bolotin DA, Hoth M, Niemeyer BA, Schultz C, 
            Zagaynova EV, Lukyanov S, Belousov VV
  TITLE     Can we see PIP(3) and hydrogen peroxide with a single probe?
  JOURNAL   Antioxid Redox Signal. 2012 Aug 1;17(3):505-12. doi: 
            10.1089/ars.2012.4574. Epub 2012 Apr 10.
  PUBMED    22369174
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/ars.2012.4574"; journalName: "Antioxid Redox Signal"; date:
            "2012-08-1- 1"; volume: "17"; issue: "3"; pages: "505-12"
COMMENT     Sequence Label: pC1-PIP-SHOW
FEATURES             Location/Qualifiers
     source          1..1965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             532..1965
                     /codon_start=1
                     /product="improved fluorescent indicator for intracellular 
                     H2O2 (Bilan et al., 2013)"
                     /label=HyPer-3
                     /translation="EMASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMYL
                     HEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVPM
                     ADLAGEKLLMLEDGHCLRDQAMSAGYNSDNVYIMADKQKNGIKANFKIRHNVEDGSVQL
                     ADHYQQNTPIGDGPVLLPDNHYLSFQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELY
                     NVDGGSGGTGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICTT
                     GKLPVPWPTLVTTLGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTR
                     AEVKFEGDTLVNRIELKGIGFKEDGNILGHKLEYNGTGFCFEAGADEDTHFRATSLETL
                     RNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQLA
                     EAIRARMDGHFDKVLKQAV"
     primer_bind     1137..1158
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1267..1288)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1528..1547)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggccgcag tgattctgga gagcatcttt ctgaagcgat cccaacagaa aaagaaaaca
       61 tcacctctaa acttcaagaa gcgcctgttt ctcttgaccg tgcacaaact ctcctactat
      121 aagtatgact ttgaacgtgg gagaagaggc agtaagaagg gttcaataga tgttgagaag
      181 atcacttgtg ttgaaacagt ggttcctgaa aaaaatcctc ctccagaaag acagattccg
      241 agaagaggtg aagagtccag cgaaatggag caaatttcaa tcattgaaag gttcccttat
      301 cccttccagg ttgtatatga tgaagggcct ctctacgtct tctccccaac tgaagaacta
      361 aggaagcggt ggattcacca gctcaaaaac gtaatccggt acaacagtga tctggttcag
      421 aaatatcacc cttgcttctg gatcgatggg cagtatctct gctgctctca gacagccaaa
      481 aatgctatgg gctgccaaat tttggagaac ggggatccac cggtcgccac cgagatggca
      541 agccagcagg gcgagacgat gtccggaccg ctgcacattg gtttgattcc cacagttgga
      601 ccgtacctgc taccgcatat tatccctatg ctgcaccaga cctttccaaa gctggaaatg
      661 tatctgcatg aagcacagac ccaccagtta ctggcgcaac tggacagcgg caaactcgat
      721 tgcgtgatcc tcgcgctggt gaaagagagc gaagcattca ttgaagtgcc gttgtttgat
      781 gagccaatgt tgctggctat ctatgaagat cacccgtggg cgaaccgcga atgcgtaccg
      841 atggccgatc tggcagggga aaaactgctg atgctggaag atggtcactg tttgcgcgat
      901 caggcaatgt ccgccggcta caacagcgac aacgtctata tcatggccga caagcagaag
      961 aacggcatca aggccaactt caagatccgc cacaacgtcg aggacggcag cgtgcagctc
     1021 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1081 cactacctga gcttccagtc cgtcctgagc aaagacccca acgagaagcg cgatcacatg
     1141 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaac
     1201 gtggatggcg gtagcggtgg caccggcagc aagggcgagg agctgttcac cggggtggtg
     1261 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1321 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
     1381 ctgcccgtgc cctggcccac cctcgtgacc accctcggct acggcctgaa gtgcttcgcc
     1441 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1501 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1561 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcgg cttcaaggag
     1621 gacggcaaca tcctggggca caagctggag tacaacggca ccggtttctg ttttgaagcc
     1681 ggggcggatg aagatacaca cttccgcgcg accagcctgg aaactctgcg caacatggtg
     1741 gcggcaggta gcgggatcac tttactgcca gcgctggctg tgccgccgga gcgcaaacgc
     1801 gatggggttg tttatctgcc gtgcattaag ccggaaccac gccgcactat tggcctggtt
     1861 tatcgtcctg gctcaccgct gcgcagccgc tatgagcagc tggcagaggc catccgcgca
     1921 agaatggatg gccatttcga taaagtttta aaacaggcgg tttaa
//
