LOCUS       sequence-69557-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     Strehler unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EGFP-hCALML3
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..44
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..80
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 agtccggact cagatcctac ccctatgatg tgcctgacta tgcccaccac caccaccacc
       61 acctggtgcc caggggcagc agatctcgag ctcaagcttc gaattctgca gtcgacggta
      121 cccacaggaa acagaccatg gccgaccagc tgactgagga gcaggtcaca gaattcaagg
      181 aggccttctc cctgtttgac aaggatgggg acggctgcat caccacccgc gagctgggca
      241 cggtcatgcg gtccctgggc cagaacccca cggaggccga gctgcgggac atgatgagtg
      301 agatcgaccg ggacggcaac ggcaccgtgg acttccccga gttcctgggc atgatggcca
      361 ggaagatgaa ggacacggac aacgaggagg agatccgcga ggccttccgc gtgttcgaca
      421 aggacggcaa cggcttcgtc agcgccgccg agctgcgaca cgtcatgacc cggctggggg
      481 agaagctgag tgacgaggag gtggacgaga tgatccgggc cgcggacacg gacggagacg
      541 gacaggtgaa ctacgaggag tttgtccgtg tgctggtgtc caagtgaggc cggcgcccac
      601 catgctcctg ggcgcccacg cggcccacag ggcaagaacc cgggggatcc accggatcta
      661 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
      721 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      781 ttattgcagc ttataatggt tacaaataaa gcaatagcat caca
//
