LOCUS       sequence-69559-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Goellner GM, DeMarco SJ, Strehler EE
  TITLE     Characterization of PISP, a novel single-PDZ protein that binds to 
            all plasma membrane Ca2+-ATPase b-splice variants.
  JOURNAL   Ann N Y Acad Sci. 2003 Apr;986:461-71.
  PUBMED    12763866
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Ann N Y 
            Acad Sci."; date: "2003-04"; volume: "986"; pages: "461-71"
COMMENT     Sequence Label: pCMV-tag2B-PISP/AIPP1
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..121
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(577..593)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(578..594)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(649..668)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(650..668)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 accgtcagat ccgctagcga ttacgccaag ctcgaaatta accctcacta aagggaacaa
       61 aagctggagc tccaccgcgg tggcggccgc caccatggat tacaaggatg acgacgataa
      121 gagcccgggc ggatccgaca gccggattcc ttatgatgac tacccggtgg ttttcttgcc
      181 tgcctatgag aatcctccag catggattcc tcctcatgag agggtacacc acccggacta
      241 caacaatgag ttgacccagt ttctgccccg aaccatcaca ctgaagaagc ctcctggagc
      301 tcagttggga tttaacatcc gaggaggaaa ggcctcccag ctaggcatct tcatctccaa
      361 ggtgattcct gactctgatg cacatagagc aggactgcag gaaggggacc aagttctagc
      421 tgtgaatgat gtggatttcc aagatattga gcacagcaag gctgttgaga tcctgaagac
      481 agctcgtgaa atcagcatgc gtgtgcgctt ctttccctac aattatcatc gccaaaaaga
      541 gaggactgtg cactaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg
      601 gcccggtacc ttaattaatt aaggtaccag gtaagtgtac ccaattcgcc ctatagtgag
      661 tcgtattaca attcactcga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      721 agatccaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
      781 agattcacag tcccaaggct catttcaggc ccctcagtcc tcacagtctg ttcatgatca
      841 taatcag
//
