LOCUS       sequence-69583-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRXTN is a modified version of pRX-tight Puro (part of the two 
            plasmid Tet-ON system from Clontech) encoding NAT instead of PAC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Kochupurakkal BS, Iglehart JD
  TITLE     Nourseothricin N-acetyl transferase: a positive selection marker for
            Mammalian cells.
  JOURNAL   PLoS One. 2013 Jul 4;8(7):e68509. doi: 10.1371/journal.pone.0068509.
            Print 2013.
  PUBMED    23861913
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-4"; volume: "8(7):e68509. doi"; pages: " 
            10.1371/journal.pone.0068509. Print 2013"
COMMENT     Sequence Label: pRXTN
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..607
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 ttcctcatct ccgggccttt cgacctgcag tatcatgggc accaccctgg acgacacggc
       61 ttaccggtac cgcaccagtg tcccggggga cgccgaggcc atcgaggcac tggatgggtc
      121 cttcaccacc gacaccgtct tccgcgtcac cgccaccggg gacggcttca ccctgcggga
      181 ggtgccggtg gacccgcccc tgaccaaggt gttccccgac gacgaatcgg acgacgaatc
      241 ggacgacggg gaggacggcg acccggactc ccggacgttc gtcgcgtacg gggacgacgg
      301 cgacctggcg ggcttcgtgg tcgtctcgta ctccggctgg aaccgccggc tgaccgtcga
      361 ggacatcgag gtcgccccgg agcaccgggg gcacggggtc gggcgcgcgt tgatggggct
      421 cgcgacggag ttcgcccgcg agcggggcgc cgggcacctc tggctggagg tcaccaacgt
      481 caacgcaccg gcgatccacg cgtaccggcg gatggggttc accctctgcg gcctggacac
      541 cgccctgtac gacggcaccg cctcggacgg cgagcaggcg ctctacatga gcatgccctg
      601 cccctaagat aaccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
      661 tcggcgtctc gagatatcag tggtccaggc tctagttttg actcaacaat atcaccagct
      721 gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa
      781 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
      841 caagg
//
