LOCUS       sequence-69585-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRXTN is a modified version of pRX-tight Puro (part of the two 
            plasmid Tet-ON system from Clontech) encoding NAT instead of PAC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Kochupurakkal BS, Iglehart JD
  TITLE     Nourseothricin N-acetyl transferase: a positive selection marker for
            Mammalian cells.
  JOURNAL   PLoS One. 2013 Jul 4;8(7):e68509. doi: 10.1371/journal.pone.0068509.
            Print 2013.
  PUBMED    23861913
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-07-4"; volume: "8(7):e68509. doi"; pages: " 
            10.1371/journal.pone.0068509. Print 2013"
COMMENT     Sequence Label: pRXTN
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        60..374
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    104..122
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    139..157
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    175..193
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    211..229
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    246..264
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    282..300
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     347..371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        430..929
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(465..488)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     862..881
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt gaagatctga ggccctttcg tcttcactcg
       61 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      121 gaacgatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
      181 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
      241 gtttatccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
      301 acgtatgtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
      361 accgtcagat cgcctggaga aggatccgcg gccgcgccgg ctctagatcg cgaacgcgtg
      421 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
      481 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
      541 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
      601 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
      661 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
      721 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
      781 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
      841 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
      901 tctcctcttc ctcatctccg ggcctttcga cctgcagtta tcatgggcac caccctggac
      961 gacacggctt
//
