LOCUS       sequence-69588-e        1437 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Peroxide-insensitive version of HyPer to be used as a pH-control. 
            Can be applied as a pH sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Belousov VV, Fradkov AF, Lukyanov KA, Staroverov DB, Shakhbazov KS, 
            Terskikh AV, Lukyanov S
  TITLE     Genetically encoded fluorescent indicator for intracellular hydrogen
            peroxide.
  JOURNAL   Nat Methods. 2006 Apr;3(4):281-6.
  PUBMED    16554833
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2006-04"; volume: "3(4)"; pages: "281-6"
COMMENT     Sequence Label: pC1-HyPer-C199S
FEATURES             Location/Qualifiers
     source          1..1437
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1437
                     /codon_start=1
                     /product="improved fluorescent indicator for intracellular 
                     H2O2 (Bilan et al., 2013)"
                     /label=HyPer-3
                     /translation="MEMASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMY
                     LHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVP
                     MADLAGEKLLMLEDGHSLRDQAMSAGYNSDNVYIMADKQKNGIKANFKIRHNVEDGSVQ
                     LADHYQQNTPIGDGPVLLPDNHYLSFQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDEL
                     YNVDGGSGGTGSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKLICT
                     TGKLPVPWPTLVTTLGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKT
                     RAEVKFEGDTLVNRIELKGIGFKEDGNILGHKLEYNGTGFCFEAGADEDTHFRATSLET
                     LRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQL
                     AEAIRARMDGHFDKVLKQAV"
     primer_bind     609..630
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(739..760)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1000..1019)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggagatgg caagccagca gggcgagacg atgtccggac cgctgcacat tggtttgatt
       61 cccacagttg gaccgtacct gctaccgcat attatcccta tgctgcacca gacctttcca
      121 aagctggaaa tgtatctgca tgaagcacag acccaccagt tactggcgca actggacagc
      181 ggcaaactcg attgcgtgat cctcgcgctg gtgaaagaga gcgaagcatt cattgaagtg
      241 ccgttgtttg atgagccaat gttgctggct atctatgaag atcacccgtg ggcgaaccgc
      301 gaatgcgtac cgatggccga tctggcaggg gaaaaactgc tgatgctgga agatggtcac
      361 tctttgcgcg atcaggcaat gtccgccggc tacaacagcg acaacgtcta tatcatggcc
      421 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacgt cgaggacggc
      481 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      541 ctgcccgaca accactacct gagcttccag tccgtcctga gcaaagaccc caacgagaag
      601 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      661 gagctgtaca acgtggatgg cggtagcggt ggcaccggca gcaagggcga ggagctgttc
      721 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      781 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gctgatctgc
      841 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctcgg ctacggcctg
      901 aagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      961 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1021 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1081 ggcttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacgg caccggtttc
     1141 tgttttgaag ccggggcgga tgaagataca cacttccgcg cgaccagcct ggaaactctg
     1201 cgcaacatgg tggcggcagg tagcgggatc actttactgc cagcgctggc tgtgccgccg
     1261 gagcgcaaac gcgatggggt tgtttatctg ccgtgcatta agccggaacc acgccgcact
     1321 attggcctgg tttatcgtcc tggctcaccg ctgcgcagcc gctatgagca gctggcagag
     1381 gccatccgcg caagaatgga tggccatttc gataaagttt taaaacaggc ggtttaa
//
