LOCUS       sequence-69595-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ecDHFR based destable domain function at low temperature for 
            regulating protein stability in Caenorhabditis elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Cho U, Zimmerman SM, Chen LC, Owen E, Kim JV, Kim SK, Wandless TJ
  TITLE     Rapid and Tunable Control of Protein Stability in Caenorhabditis 
            elegans Using a Small Molecule.
  JOURNAL   PLoS One. 2013 Aug 22;8(8):e72393. doi: 
            10.1371/journal.pone.0072393.
  PUBMED    23991108
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-08-22"; volume: "8(8):e72393. doi"; pages: " 
            10.1371/journal.pone.0072393"
COMMENT     Sequence Label: pBMN ltDHFR(DD)-YFP
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..502)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDHVIGMENAMPWSLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSADEAIAACGDVPEIMVIGGGRVYEQFL
                     PKAQKLYLTHIDAEVGGDTHFPDYEPDDWGSVFSEFHDADAQNSHSYCFVILGRR"
     misc_feature    517..891
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(563..579)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(917..933)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 gctcctcgcc cttgctcacg catgctcgcc gccccagaat cacaaagcaa tagctgtgag
       61 agttctgcgc atcagcatcg tggaattcgc tgaataccga tccccagtca tccggctcgt
      121 aatccgggaa atgggtgtcg cctcccactt ctgcgtcgat atgcgtcaga tacagttttt
      181 gcgcttttgg caagaactgc tcataaacgc gaccgccgcc aatcaccatg atttctggta
      241 cgtcaccaca cgccgcgatg gcttcatccg ccgacttcac ccacgttacg cgatcgtccg
      301 tactcggttg actgctgagg ataatgtttt tgcgtcctgg caacggacga ccgattgatt
      361 cccaggtatg gcggcccata atcacgggtt tatttaaggt gttgcgttta aaccaggcga
      421 gatcggcagg caggctccac ggcatggcgt tttccatgcc gataacgtga tctaccgcta
      481 acgccgcaat cagactgatc atggtggcgg atccggcagt ctagaggatg gtccaccccc
      541 ggggtcggca gccttcacgt gggcggcgtg tatccaagct gcgatgccgt ctactttgag
      601 ggcggtgggg gtggtcagca ggactgtgta aggtcctttc cagcgaggtt ctaggttctt
      661 agtctggtgt cggcggaccc acactgtgtc gccgactcgg taagggtgag gtaccaccgg
      721 tcggtccagt tgttcttggt aggctgccgc cagaggtctc cagacttcgt gctggactaa
      781 gtagagagcc tgtaagtgag cttggagaga ggggctgtta gtaactcttg tcatgtcagg
      841 gtcagggaag tttacaaggg gcgggggtgc cccatataag atctcatatg gccatatggg
      901 ggcgcctaga gaaggagtga gggctggata aagggagga
//
