LOCUS       sequence-69597-e         525 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ecDHFR based destable domain function at low temperature for 
            regulating protein stability in Caenorhabditis elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Cho U, Zimmerman SM, Chen LC, Owen E, Kim JV, Kim SK, Wandless TJ
  TITLE     Rapid and Tunable Control of Protein Stability in Caenorhabditis 
            elegans Using a Small Molecule.
  JOURNAL   PLoS One. 2013 Aug 22;8(8):e72393. doi: 
            10.1371/journal.pone.0072393.
  PUBMED    23991108
REFERENCE   2  (bases 1 to 525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-08-22"; volume: "8(8):e72393. doi"; pages: " 
            10.1371/journal.pone.0072393"
COMMENT     Sequence Label: pBMN YFP-ltDHFR(DD)
FEATURES             Location/Qualifiers
     source          1..525
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..498
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDHVIGMENAMPWNLPADLAWFKRNTLDKPVIMGRHT
                     WESIGRPLPGRKDIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDGWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 gacgagctgt acaagctcga gatcagtctg attgcggcgt tagcggtaga tcacgttatc
       61 ggcatggaaa acgccatgcc gtggaacctg cctgccgatc tcgcctggtt taaacgcaac
      121 accttagata aacccgtgat tatgggccgc catacctggg aatcaatcgg tcgtccgttg
      181 ccaggacgca aagatattat cctcagcagt caaccgagta cggacgatcg cgtaacgtgg
      241 gtgaagtcgg tggatgaagc catcgcggcg tgtggtgacg taccagaaat catggtgatt
      301 ggcggcggtc gcgtttatga acagttcttg ccaaaagcgc aaaaactgta tctgacgcat
      361 atcgacgcag aagtggaagg cgacacccat ttcccggatt acgagccgga tggctgggaa
      421 tcggtattca gcgaattcca cgatgctgat gcgcagaact ctcacagcta ttgctttgag
      481 attctggagc ggcgatgacc gcggccgcta cgtaaattcc gcccc
//
