LOCUS       sequence-69598-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Qian H, Rogers MS, Schleucher J, Edlund U, Strehler EE, Sethson I
  TITLE     Sequential assignment of 1H, 15N, 13C resonances and secondary 
            structure of human calmodulin-like protein determined by NMR 
            spectroscopy.
  JOURNAL   Protein Sci. 1998 Nov;7(11):2421-30.
  PUBMED    9828009
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "1998-11"; volume: "7(11)"; pages: "2421-30"
COMMENT     Sequence Label: pGEMEX174-hCALML3
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             520..542
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(573..592)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(574..592)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(684..701)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atccccgggt tcttgccctg tgggccgcgt gggcgcccag gagcatggtg ggcgccggcc
       61 tcacttggac accagcacac ggacaaactc ctcgtagttc acctgtccgt ctccgtccgt
      121 gtccgcggcc cggatcatct cgtccacctc ctcgtcactc agcttctccc ccagccgggt
      181 catgacgtgt cgcagctcgg cggcgctgac gaagccgttg ccgtccttgt cgaacacgcg
      241 gaaggcctcg cggatctcct cctcgttgtc cgtgtccttc atcttcctgg ccatcatgcc
      301 caggaactcg gggaagtcca cggtgccgtt gccgtcccgg tcgatctcac tcatcatgtc
      361 ccgcagctcg gcctccgtgg ggttctggcc cagggaccgc atgaccgtgc ccagctcgcg
      421 ggtggtgatg cagccgtccc catccttgtc aaacagggag aaggcctcct tgaattctgt
      481 gacctgctcc tcagtcagct ggtcggccat gtatgtatat ctccttctta aagttaaaca
      541 aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat taatttcgcg
      601 ggatcgagat ctcccgggct gcattaatga atcggccaac gcgcggggag aggcggtttg
      661 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      781 aacgcaggaa agaacatgtg agcaa
//
