LOCUS       sequence-69608-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vivo expression of Cas9 or generation of Cas9 mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, 
            Montague TG, Zimmerman S, Richter C, Schier AF
  TITLE     Efficient mutagenesis by Cas9 protein-mediated oligonucleotide 
            insertion and large-scale assessment of single-guide RNAs.
  JOURNAL   PLoS One. 2014 May 29;9(5):e98186. doi: 
            10.1371/journal.pone.0098186. eCollection 2014.
  PUBMED    24873830
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-29"; volume: "9(5):e98186. doi"; pages: " 
            10.1371/journal.pone.0098186. eCollection 2014"
COMMENT     Sequence Label: pCS2-Cas9
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..51)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgtaatacga ctcactatag ttctagatta caccttcctc ttcttcttgg ggtcagccct
       61 gctgtctcca ccgagctgag agaggtcgat tcttgtttca tagagccccg taattgactg
      121 atgaatcagt gtggcgtcca ggacctcctt tgtagaggtg taccgctttc tgtctatggt
      181 ggtgtcgaag tacttgaagg ctgcaggcgc gcccaagttg gtcagagtaa acaagtggat
      241 aatgttttct gcctgctccc tgatgggctt atccctgtgc ttattgtaag cagaaagcac
      301 cttatcgagg ttagcgtcgg cgaggatcac tcttttggag aattcgctta tttgctcgat
      361 gatctcatca aggtagtgtt tgtgttgttc cacgaacagc tgcttctgct cattatcttc
      421 gggagaccct ttgagctttt catagtggct ggccagatac aagaaattaa cgtatttaga
      481 gggcagtgcc agctcgttac ctttctgcag ctcgcccgca ctagcgagca ttcgtttccg
      541 gccgttttca agctcaaaga gagagtactt gggaagctta atgatgaggt cttttttgac
      601 ctctttatat cctttcgcct cgagaaagtc gatggggttt ttttcgaagc ttgatcgctc
      661 catgattgtg atgcccagca gttccttgac gcttttgagt tttttagact tccctttctc
      721 cactttggcc acaaccagta cactgtaagc gactgtagga gaatcgaatc cgccgtattt
      781 cttggggtcc caatcttttt tgcgtgcgat cagcttgtcg ctgttccttt tcgggaggat
      841 actttccttg gagaagcctc cggtctgtac ttcggtcttt ttaacgatgt tcacctgcgg
      901 catggacagg accttccgga ctgtcgcgaa atccctaccc ttgtcccaca cgatttctcc
      961 tgtttctccg tttggttcga taagtggtcg
//
