LOCUS       sequence-69612-e        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro expression  and purification of Cas9 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, 
            Montague TG, Zimmerman S, Richter C, Schier AF
  TITLE     Efficient mutagenesis by Cas9 protein-mediated oligonucleotide 
            insertion and large-scale assessment of single-guide RNAs.
  JOURNAL   PLoS One. 2014 May 29;9(5):e98186. doi: 
            10.1371/journal.pone.0098186. eCollection 2014.
  PUBMED    24873830
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-29"; volume: "9(5):e98186. doi"; pages: " 
            10.1371/journal.pone.0098186. eCollection 2014"
COMMENT     Sequence Label: pET-28b-Cas9-His
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             18..40
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg acaagaagta
       61 ctccattggg ctcgatatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta
      121 caaggtgccg agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa
      181 gaacctcatt ggcgccctcc tgttcgactc cggggagacg gccgaagcca cgcggctcaa
      241 aagaacagca cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat
      301 ctttagtaat gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt
      361 tttggtggag gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga
      421 ggtggcgtac catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag
      481 tactgataag gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg
      541 gggacacttc ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt
      601 tatccaactg gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg
      661 agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct
      721 catcgcacag ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc
      781 actcgggctg acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca
      841 actgagcaaa gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca
      901 gtacgcagac ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat
      961 tctgcgagtg aacacggaga tcaccaaagc tccgctgagc gctagtatga
//
