LOCUS       sequence-69616-e        1431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Hydrogen peroxide insensitive version of HyPerRed to be used as a pH
            control in HyPer experiments..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Ermakova YG, Bilan DS, Matlashov ME, Mishina NM, Markvicheva KN, 
            Subach OM, Subach FV, Bogeski I, Hoth M, Enikolopov G, Belousov VV
  TITLE     Red fluorescent genetically encoded indicator for intracellular 
            hydrogen peroxide.
  JOURNAL   Nat Commun. 2014 Oct 21;5:5222. doi: 10.1038/ncomms6222.
  PUBMED    25330925
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-10-21"; volume: "5:5222. doi"; pages: " 
            10.1038/ncomms6222"
COMMENT     Sequence Label: pC1-HyPer-Red-C199S
FEATURES             Location/Qualifiers
     source          1..1431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..381,382..390,391..1116,1117..1125,1126..1428)
                     /codon_start=1
                     /product="red fluorescent genetically encoded indicator for
                     H2O2 ()"
                     /label=HyPerRed
                     /translation="MEMASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMY
                     LHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVP
                     MADLAGEKLLMLEDGHSLRDQAMPRTVSERMYPEDGALKSEIKKGLRLKDGGHYAAEVK
                     TTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQCERAEGRHSTGGMDELYKGGTGGS
                     LVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPL
                     PFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSL
                     QDGVFIYKVKLRGTNFPPDGPVMQKKTMGWEAAGVGFCFEAGADEDTHFRATSLETLRN
                     MVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQLAEA
                     IRARMDGHFDKVLKQAV"
     primer_bind     1080..1099
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggagatgg cgagccagca gggcgagacg atgtccggac cgctgcacat tggtttgatt
       61 cccacagttg gaccgtacct gctaccgcat attatcccta tgctgcacca gacctttcca
      121 aagctggaaa tgtatctgca tgaagcacag acccaccagt tactggcgca actggacagc
      181 ggcaaactcg attgcgtgat cctcgcgctg gtgaaagaga gcgaagcatt cattgaagtg
      241 ccgttgtttg atgagccaat gttgctggct atctatgaag atcacccgtg ggcgaaccgc
      301 gaatgcgtac cgatggccga tctggcaggg gaaaaactgc tgatgctgga agatggtcac
      361 tctttgcgcg atcaggcaat gccccgcacg gtttccgagc ggatgtaccc cgaggacggc
      421 gccctgaaga gcgagatcaa gaaggggctg aggctgaagg acggcggcca ctacgccgcc
      481 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc
      541 gacatcaagt tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtgcgaa
      601 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg aggtacaggc
      661 gggagtctgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      721 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      781 gagggccgcc cctacgaggc ctttcagacc gctaagctga aggtgaccaa gggtggcccc
      841 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacatt
      901 aagcacccag ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaggtgg
      961 gagcgcgtga tgaacttcga ggacggcggc attattcacg ttaaccagga ctcctccctg
     1021 caggacggcg tattcatcta caaggtgaag ctgcgcggca ccaacttccc ccccgacggc
     1081 cccgtaatgc agaagaagac catgggctgg gaggctgccg gggtgggttt ctgttttgaa
     1141 gccggggcgg atgaagatac acacttccgc gcgaccagcc tggaaactct gcgcaacatg
     1201 gtggcggcag gtagcgggat cactttactg ccagcgctgg ctgtgccgcc ggagcgcaaa
     1261 cgcgatgggg ttgtttatct gccgtgcatt aagccggaac cacgccgcac tattggtctg
     1321 gtttatcgtc ctggctcacc gctgcgcagc cgctatgagc agctggcaga ggccatccgc
     1381 gcaagaatgg atggccattt cgataaagtt ttaaaacagg cggtttaatg a
//
