LOCUS       sequence-69618-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-VP64 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Maeder ML, Linder SJ, Cascio VM, Fu Y, Ho QH, Joung JK
  TITLE     CRISPR RNA-guided activation of endogenous human genes.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598.
  PUBMED    23892898
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598."
COMMENT     Sequence Label: pSL690
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agagatccgc ggccgctaat acgactcact
       61 atagggagag ccgccaccat ggataagaaa tactcaatag gcttagctat cggcacaaat
      121 agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt
      181 ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct tttatttgac
      241 agtggagaga cagcggaagc gactcgtctc aaacggacag ctcgtagaag gtatacacgt
      301 cggaagaatc gtatttgtta tctacaggag attttttcaa atgagatggc gaaagtagat
      361 gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa gaagcatgaa
      421 cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa atatccaact
      481 atctatcatc tgcgaaaaaa attggtagat tctactgata aagcggattt gcgcttaatc
      541 tatttggcct tagcgcatat gattaagttt cgtggtcatt ttttgattga gggagattta
      601 aatcctgata atagtgatgt ggacaaacta tttatccagt tggtacaaac ctacaatcaa
      661 ttatttgaag aaaaccctat taacgcaagt ggagtagatg ctaaagcgat tctttctgca
      721 cgattgagta aatcaagacg attagaaaat ctcattgctc agctccccgg tgagaagaaa
      781 aatggcttat ttgggaatct cattgctttg tcattgggtt tgacccctaa ttttaaatca
      841 aattttgatt tggcagaaga tgctaaatta cagctttcaa a
//
