LOCUS       sequence-69619-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-VP64 fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Maeder ML, Linder SJ, Cascio VM, Fu Y, Ho QH, Joung JK
  TITLE     CRISPR RNA-guided activation of endogenous human genes.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598.
  PUBMED    23892898
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598."
COMMENT     Sequence Label: pSL690
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(27..176)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(207..272)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(279..299)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggtgatgg tgatgatgac cggttataac atatcgagat cgaaatcgtc cagagcatcg
       61 gagccgagca tgtccagatc aaagtcatcc aatgcgtcgc ttcccaacat atccaggtca
      121 aagtcatcga gggcatcaga acccagcatg tcgagatcga aatcgtccag cgcgtcggcg
      181 cgcccgcttc caccgcctcc tgcagccttg tcatcgtcat ccttgtaatc gatgtcatga
      241 tctttataat caccgtcatg gtctttgtag tcgctcgaga ctttcctctt cttcttggga
      301 gaaccgtcac ctcctagctg actcaaatca atgcgtgttt cataaagacc agtgatggat
      361 tgatggataa gagtggcatc taaaacttct tttgtagacg tatatcgttt acgatcaatt
      421 gttgtatcaa aatatttaaa agcagcggga gctccaagat tcgtcaacgt aaataaatga
      481 ataatatttt ctgcttgttc acgtattggt ttgtctctat gtttgttata tgcactaaga
      541 actttatcta aattggcatc tgctaaaata acacgcttag aaaattcact gatttgctca
      601 ataatctcat ctaaataatg cttatgctgc tccacaaaca attgtttttg ttcgttatct
      661 tctggactac ccttcaactt ttcataatga ctagctaaat ataaaaaatt cacatatttg
      721 cttggcagag ccagctcatt tcctttttgt aattctccgg cactagccag catccgttta
      781 cgaccgtttt ctaactcaaa aagactatat ttaggtagtt taatgattaa gtctttttta
      841 acttccttat atcctttagc ttctaaaaag tcaatcggat ttttttcaaa ggaacttctt
      901 tccataattg tgatccctag taactcttta acggatttta actt
//
