LOCUS       sequence-69621-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mammalian cell codon-optimized dCas9-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Maeder ML, Linder SJ, Cascio VM, Fu Y, Ho QH, Joung JK
  TITLE     CRISPR RNA-guided activation of endogenous human genes.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598.
  PUBMED    23892898
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2598."
COMMENT     Sequence Label: pMLM3705
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      73..82
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agagatccgc ggccgctaat acgactcact
       61 atagggagag ccgccaccat ggataaaaag tattctattg gtttagccat cggcactaat
      121 tccgttggat gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa atttaaggtg
      181 ttggggaaca cagaccgtca ttcgattaaa aagaatctta tcggtgccct cctattcgat
      241 agtggcgaaa cggcagaggc gactcgcctg aaacgaaccg ctcggagaag gtatacacgt
      301 cgcaagaacc gaatatgtta cttacaagaa atttttagca atgagatggc caaagttgac
      361 gattctttct ttcaccgttt ggaagagtcc ttccttgtcg aagaggacaa gaaacatgaa
      421 cggcacccca tctttggaaa catagtagat gaggtggcat atcatgaaaa gtacccaacg
      481 atttatcacc tcagaaaaaa gctagttgac tcaactgata aagcggacct gaggttaatc
      541 tacttggctc ttgcccatat gataaagttc cgtgggcact ttctcattga gggtgatcta
      601 aatccggaca actcggatgt cgacaaactg ttcatccagt tagtacaaac ctataatcag
      661 ttgtttgaag agaaccctat aaatgcaagt ggcgtggatg cgaaggctat tcttagcgcc
      721 cgcctctcta aatcccgacg gctagaaaac ctgatcgcac aattacccgg agagaagaaa
      781 aatgggttgt tcggtaacct tatagcgctc tcactaggcc tgacaccaaa ttttaagtcg
      841 aacttcgact tagctgaaga tgccaaattg cagcttagta aggacacgta cgatgacgat
      901 ctcgacaatc tactggcaca aattggagat cagtatgcgg acttattttt ggctgccaaa
      961 a
//
