LOCUS       sequence-69623-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Bennett RD, Strehler EE
  TITLE     Calmodulin-like protein enhances myosin-10 translation.
  JOURNAL   Biochem Biophys Res Commun. 2008 May 2;369(2):654-9. doi: 
            10.1016/j.bbrc.2008.02.056. Epub 2008 Feb 22.
  PUBMED    18295593
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2008.02.056"; journalName: "Biochem Biophys Res 
            Commun"; date: "2008-05-2- 2"; volume: "369"; issue: "2"; pages: 
            "654-9"
COMMENT     Sequence Label: pcDNA3.1-6His-MyoX
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..113
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             117..149
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(153..161,162..176)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      527..536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
       61 tagcgtttaa acttaagctt accatggggg gttctcatca tcatcatcat catggtatgg
      121 ctagcatgac tggtggacag caaatgggtc gggatctgta cgacgatgac gataaggtac
      181 catggataac ttcttcaccg agggaacacg ggtctggctg agagaaaatg gccagcattt
      241 tccaagtact gtaaattcct gtgcagaagg catcgtcgtc ttccggacag actatggtca
      301 ggtattcact tacaagcaga gcacaattac ccaccagaag gtgactgcta tgcaccccac
      361 gaacgaggag ggcgtggatg acatggcgtc cttgacagag ctccatggcg gctccatcat
      421 gtataactta ttccagcggt ataagagaaa tcaaatatat acctacatcg gctccatcct
      481 ggcctccgtg aacccctacc agcccatcgc cgggctgtac gagcctgcca ccatggagca
      541 gtacagccgg cgccacctgg gcgagctgcc cccgcacatc ttcgccatcg ccaacgagtg
      601 ctaccgctgc ctgtggaagc gctacgacaa ccagtgcatc ctcatcagtg gtgaaagtgg
      661 ggcaggtaaa accgaaagca ctaaattgat cctcaagttt ctgtcagtca tcagtcaaca
      721 gtctttggaa ttgtccttaa aggagaagac atcctgtgtt gaacgagcta ttcttgaaag
      781 cagccccatc atggaagctt tcgg
//
