LOCUS       sequence-69626-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  engineered receptor improves heteromeric receptor expression and 
            sensitivity to IVM..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Frazier SJ, Cohen BN, Lester HA
  TITLE     An Engineered GluCl Channel for Sensitive, Consistent Neuronal 
            Silencing by Ivermectin.
  JOURNAL   J Biol Chem. 2013 May 29.
  PUBMED    23720773
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2013 May 29."
COMMENT     Sequence Label: pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F...
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..23)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(33..74)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(348..599)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(390..411)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     739..758
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     998..1019
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 actcaatggt gatggtgatg atgaccggta cgcgtagaat cgagaccgag gagagggtta
       61 gggataggct taccttcgaa ccgcgggccc tctagactcg agcggccgcc actgtgctgg
      121 atatctgcag aattgccctt gctagcctac accagggact cgggggtgct ggcgttagcg
      181 ctcatgatca ggcaggacac ccagtagatc acgttgaagg ccaggaaggc caggggcacc
      241 acgaagcggg cgtagaagtc gatcttggcg ggcaggtagt cgggcttggt gaagtagcgt
      301 ctgactttgt cgcggaaggt ctctctggcc tcgaaggtgt ggcacgtctt gtacagctcg
      361 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
      421 ttctcgttgg ggtctttgct cagcttggac tggtagctca ggtagtggtt gtcgggcagc
      481 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
      541 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
      601 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
      661 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
      721 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
      781 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
      841 tagcgggcga agcactgcag gccgtagccg aaggtggtca cgagggtggg ccagggcacg
      901 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
      961 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     1021 atgggcacca cccc
//
