LOCUS       sequence-6964-ex       13402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13402)
  AUTHORS   Loftus SK, Larson DM, Watkins-Chow D, Church DM, Pavan WJ
  TITLE     Generation of RCAS vectors useful for functional genomic analyses.
  JOURNAL   DNA Res. 2001 Oct 31. 8(5):221-6.
  PUBMED    11759842
REFERENCE   2  (bases 1 to 13402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res. 
            2001 Oct 31. 8(5):221-6."
COMMENT     Sequence Label: RCASBP-Y NHA
FEATURES             Location/Qualifiers
     source          1..13402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7048..7074
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    7084..7208
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7233..7238,7239..7256,7257..7263)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(7383..7402)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     8499..8518
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8661..8785)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        8940..9201
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(9792..9814)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9803..9819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9827..9833,9834..9851,9852..9857))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(9900..9918)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9986..10090
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10091..10159,10160..10951)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10309..10328)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11122..11710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11611..11630
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11864..11881
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11896..12036
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11962..11981)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12122..12144
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(12138..12329)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        join(13174..13402,1..33)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gccatttgac cattcaccac attggtgtgc acctgggttg atggccggac cgttgattcc
       61 ctgacgacta cgagcacctg catgaagcag aaggcttcat ttggtgaccc cgacgtgata
      121 gttagggaat agtggtcggc cacagacggc gtggcgatcc tgtctccatc cgtctcgtct
      181 atcgggaggc gagttcgatg accctggtgg agggggctgc ggcttaggga ggcagaagct
      241 gagtaccgtc ggagggagct ccagggcccg gagcgactga cccctgccga gaactcagag
      301 ggtcgtcgga agacggagag tgagcccgac gaccacccca ggcacgtctt tggtcggcct
      361 gcggatcaag catggaagcc gtcattaagg tgatttcgtc cgcgtgtaaa acctattgcg
      421 ggaaaatctc tccttctaag aaggaaatag gggccatgtt gtccctgtta caaaaggaag
      481 ggttgcttat gtctccctca gatttatatt ctccggggtc ctgggatccc atcactgcgg
      541 cgctctccca gcgggcaatg gtacttggaa aatcgggaga gttaaaaacc tggggattgg
      601 ttttgggggc attgaaggcg gctcgagagg aacaggttac atctgagcaa gcaaagtttt
      661 ggttgggatt agggggaggg agggtctctc ccccaggtcc ggagtgcatc gagaaaccag
      721 ctacggagcg gcgaatcgac aaaggggagg aggtgggaga aacaactgtg cagcgagatg
      781 cgaagatggc gccagaggaa gcggccacac ctaaaaccgt tggcacatcc tgctatcatt
      841 gcggaacagc tgttggctgc aattgcgcca ccgccacagc ctcggcccct cctccccctt
      901 atgtggggag tggtttgtat ccttccctgg cgggggtggg agagcagcag ggccagggag
      961 ataacacgtc tcggggggcg gagcagccaa gggaggagcc agggcacgcg ggtcaggccc
     1021 ctgggccggc cctgactgac tgggcaaggg taagggagga gcttgcgagt actggtccgc
     1081 ccgtggtggc catgcctgta gtgattaaga cagagggacc cgcctggacc cctctggagc
     1141 caaaattgat cacaagactg gctgatacgg tcaggaccaa gggcttacga tccccgatca
     1201 ctatggcaga agtggaagcg ctcatgtcct ccccgttgct gccgcatgac gtcacgaatc
     1261 taatgagagt gattttagga cctgccccat atgccttatg gatggacgct tggggagtcc
     1321 aactccagac ggttatagcg gcagccactc gcgacccccg acacccagcg aacggtcaag
     1381 ggcgggggga acggactaac ttggatcgat taaagggctt agctgatggg atggtgggca
     1441 acccacaggg tcaggccgca ttattaagac cgggggaatt ggttgctatt acggcgtcgg
     1501 ctctccaggc gtttagagaa gttgcccggc tggcggaacc tgcaggtcca tgggcggaca
     1561 tcacgcaggg accatctgag tcctttgttg attttgccaa tcggcttata aaggcggttg
     1621 aggggtcaga tctcccgcct tccgcgcggg ctccggtgat cattgactgc tttaggcaga
     1681 agtcacagcc agatattcag cagcttatac gggcagcacc ctccacgctg accaccccag
     1741 gagagataat caaatatgtg ctagacaggc agaagattgc ccctcttacg gatcaaggca
     1801 tagccgcggc catgtcgtct gctatccagc ccttagttat ggcagtagtc aatagagaga
     1861 gggatggaca aactgggtcg ggtggtcgtg cccgagggct ctgctacact tgtggatccc
     1921 cgggacatta tcaggcacag tgcccgaaaa aacgaaagtc aggaaacagc cgtgagcgat
     1981 gtcagctgtg tgacgggatg ggacacaacg ctaaacagtg taggaagcgg gatggcaacc
     2041 agggccaacg cccaggaaga ggtctctctt cggggccgtg gcccggccct gagcagcctg
     2101 ccgtctcgtt agcgatgaca atggaacata aagatcgccc cttggttagg gtcattctga
     2161 ctaacactgg gagtcatcca gtcaaacaac gttcggtgta tatcaccgcg ctgttggact
     2221 ccggagcgga catcactatt atttcggagg aggattggcc tactgattgg ccggtggtgg
     2281 acaccgcgaa cccacagatc catggcatag gagggggaat tcccatgcga aaatcccggg
     2341 atatgataga ggtgggggtt attaaccgag acgggtcgtt ggagcgaccc ctgctcctct
     2401 tccccgcagt cgctatggtt agagggagta tcctaggaag agattgtctg cagggcctag
     2461 ggctccgctt gacaaattta tagggagggc cactgttctt actgttgcgc tacatctggc
     2521 tattccgctc aaatggaagt cagaccgcac gcctgtgtgg attgaccagt ggccccttcc
     2581 tgaaggtaaa cttgtagcgc taacgcaatt agtggaaaaa gaattacagt taggacatat
     2641 agagccctca cttagttgtt ggaacacacc tgtttttcgt gatccggaag gcttccgggt
     2701 cttatcgctt attgcatgat ttgcgcgctg ttaacgccaa gcttgtccct tttggggccg
     2761 tccaacaggg ggcgccagtt ctctccgcgc tcccgcgtgg ctggcccctg atggtcctag
     2821 acctcaagga ttgcttcttt tctatccctc ttgcggaaca agatcgcgaa gcttttgcat
     2881 ttacgctccc ctctgtgaat aaccaggccc ccgctcgaag attccaatgg aaggtcttgc
     2941 cccaagggat gacctgttct cccactatct gtcagttggt agtgggtcag gtgctcgagc
     3001 ccttgcgact caagcaccca gctctgcgca tgttgcatta tatggacgat cttttgctag
     3061 ccgcctcaag tcatgatggg ttggaagcgg cagggaagga ggttatcggt acattggaaa
     3121 gagccgggtt cactatttcg ccggataaga tccagaggga gcccggagta caatatcttg
     3181 ggtacaagtt aggcagtacg tatgtagcac ccgtaggctt ggtagcagaa cccaggatag
     3241 ccaccttgtg ggatgttcaa aagctggtgg ggtcacttca gtggcttcgc ccagcgttag
     3301 ggatcccgcc acgactgatg ggtccctttt atgagcagtt acgagggtca gatcctaacg
     3361 aggcgaggga atggaatcta gacatgaaaa tggcctggag agagatcgta cagcttagca
     3421 ctactgctgc cttggaacga tgggaccctg cccagcctct ggaaggagcg gtcgctagat
     3481 gtgaacaggg ggcaataggg gtcctgggac agggactgtc cacacaccca aggccatgtt
     3541 tgtggttatt ctccacccaa cccaccaagg cgtttactgc ttggttagaa gtgctcaccc
     3601 ttttgattac taagctacgc gcttcggcag tgcgaacctt tggcaaggag gttgatatcc
     3661 tcctgttgcc tgcatgcttc cgggaggacc ttccgctccc ggaggggatc ctgttagcac
     3721 ttagggggtt tgcaggaaaa atcaggagta gtgacacgcc atctattttt gacattgcgc
     3781 gtccactgca tgtttctctg aaagtgaggg ttaccgacca ccctgtgccg ggacccactg
     3841 tctttaccga cgcctcctca agcacccata aaggggtggt agtctggagg gagggcccaa
     3901 ggtgggagat aaaagaaata gttgatttgg gggcaagtgt acaacaactg gaggcacgcg
     3961 ctgtggccat ggcacttctg ctgtggccga caacgcccac taatgtagtg actgactctg
     4021 cgtttgttgc gaaaatgtta ctcaagatgg gacaggaggg agtcccgtct acagcggcag
     4081 cttttatttt agaggatgcg ttaagccaaa ggtcagccat ggccgccgtt ctccacgtgc
     4141 ggagtcattc tgaagtgcca gggtttttca cagaaggaaa tgacgtggca gatagccaag
     4201 ccacctttca agcgtatccc ttgagagagg ctaaagatct tcataccgct ctccatattg
     4261 gaccccgcgc gctatccaaa gcgtgtaata tatctatgca gcaggctagg gaggttgttc
     4321 agacctgccc gcattgtaat tcagcccctg cgttggaggc cggggtaaac cctaggggtt
     4381 tgggacccct acagatatgg cagacagact ttacgcttga gcctagaatg gctccccgtt
     4441 cctggctcgc tgttactgtg gacaccgcct catcagcgat agtcgtaact cagcatggcc
     4501 gtgttacatc ggttgctgca caacatcatt gggccacggc tatcgccgtt ttgggaagac
     4561 caaaggccat aaaaacagat aacgggtcct gcttcacgtc tagatccacg cgggagtggc
     4621 tcgcgagatg ggggatagca cacaccaccg ggattccggg aaattcccag ggtcaagcta
     4681 tggtagagcg ggccaaccgg ctcctgaaag ataagatccg tgtgcttgcg gagggggacg
     4741 gctttatgaa aagaatcccc gccagcaaac agggggaact actagccaag gcaatgtatg
     4801 ccctcaatca ctttgagcgt ggtgaaaaca caaaaacacc ggtacaaaaa cactggagac
     4861 ctaccgttct tacagaagga cccccggtta aaatacgaat agagacaggg gagtgggaaa
     4921 aaggatggaa cgtgctggtc tggggacgag gttatgccgc tgtgaaaaac agggacactg
     4981 ataaggttat ttgggtaccc tctcggaaag ttaaaccgga tgtcacccaa aaggatgagg
     5041 tgactaagaa agatgaggcg agccctcttt ttgcaggcat ttctgactgg ataccctggg
     5101 aagacgagca agaaggactc caaggagaaa ccgctagcaa caagcaagaa agacccggag
     5161 aagacaccct tgctgccaac gagagttaat tatattctca ttattggtgt cctggtcttg
     5221 tgtgaggtta cgggggtaag agctgatgtc cacttactcg agcagccagg gaacctttgg
     5281 attacatggg ccaaccgtac aggccaaacg gatttttgcc tctctacaca gtcagccacc
     5341 tccccttttc aaacatgttt gataggtatc ccgtccccta tttccgaggg tgattttaag
     5401 ggatatgttt ctgatacaaa ttgcaccacc ttgggaactg atcggttagt ctcgtcagcc
     5461 gactttactg gcggacctga caacagtacc accctcactt atcggaaggt ctcatgcttg
     5521 ttgttaaagc tgaatgtctc tatgtgggat gagccacctg aactacagct gttaggttcc
     5581 cagtctctcc ctaacattac taatattgct cagatttccg gtataaccgg gggatgcgta
     5641 ggcttcagac cacaaggggt tccttggtat ctaggttggt ctagacagga ggccacgcgg
     5701 tttctcctta gacacccctc tttctctaaa tccacggaac cgtttacagt ggtgacagcg
     5761 gataggcaca atctttttat ggggagtgag tactgcggtg catatggcta cagattttgg
     5821 aacatgtata actgctcaca ggtggggcgg cagtaccgct gtggtaatgc gcgcacgccc
     5881 cgcacgggtc ttcctgaaat ccagtgtaca aggagaggag gcaaatgggt taatcaatca
     5941 caggaaatta atgagtcgga gccgttcagc tttacggtga actgtacagc tagtagtttg
     6001 ggtaatgcca gtgggtgttg cggaaaagca ggcacgattc tcccgggaaa gtgggtcgac
     6061 agcacacaag gtagtttcac caaaccaaaa gcgctaccac ccgcaatttt cctcatttgt
     6121 ggggatcgcg catggcaagg aattcccagt cgtccggtag ggggcccctg ctatttaggc
     6181 aagcttacca tgttagcacc taagcataca gatattctca aggtgcttgt caattcatcg
     6241 cggacaggta taagacgtaa acgaagcacc tcacacctgg atgatacatg ctcagatgaa
     6301 gtgcagcttt ggggtcctac agcaagaatc tttgcatcta tcctagcccc gggggtagca
     6361 gctgcgcaag ccttaagaga aattgagaga ctagcctgtt ggtccgttaa acaggctaac
     6421 ttgacaacat cactcctcgg ggacttattg gatgatgtca cgagtattcg acacgcggtc
     6481 ctgcagaacc gagcggctat tgacttcttg ctcctagctc acggccatgg ctgtgaggac
     6541 gttgccggaa tgtgctgttt caatttgagt gatcagagtg agtctataca gaagaagttc
     6601 cagctaatga aggaacatgt caataagatc ggcgtggata gcgacctaat tggaagttgg
     6661 ctgcgaggac tattcggggg aataggagaa tgggccgttc atttgctgaa aggactgctt
     6721 ttggggcttg tagttatttt gttgctagta gtgtgcctgc cttgcctttt gcaaatgtta
     6781 tgcggtaata ggagaaagat gattaataac tccatcagct accacacgga atataagaag
     6841 ctgcaaaagg cctgtgggca gcctgaaagc agaatagtat aaggcagtac atgggtggtg
     6901 gtatagcgct tgcgagtcca tcgagcaagg caggaaagac agctattggt aattgtgaaa
     6961 tacgcttttg tctgtgtgct gcaggagctg agctgactct gctggtggcc tcgcgtacca
     7021 ctgtggcatc ggcggccgca ccatgcctac ccttatgacg tgccagatta tgcctgttta
     7081 tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
     7141 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     7201 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     7261 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
     7321 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     7381 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     7441 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     7501 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     7561 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     7621 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     7681 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taagggttta ttgagaatat
     7741 gtttttcgtc tcagccaatc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     7801 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     7861 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     7921 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcgt
     7981 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt
     8041 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc
     8101 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt
     8161 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga
     8221 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa
     8281 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata
     8341 aaagagagag cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     8401 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     8461 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     8521 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     8581 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     8641 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     8701 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     8761 gttcagcttt cttgtacaaa gtggttgata aacatttaaa tatcgatgcg atgtacgggc
     8821 cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt
     8881 gtacgcggtt aggagtcccc ttaggatata gtagtttcgc ttttgcatag ggagggggaa
     8941 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     9001 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     9061 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattc
     9121 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca
     9181 ttcaccacat tggtgtgcac ctgggttgat ggccggaccg ttgattccct gacgactacg
     9241 agcacctgca tgaagcagaa ggcttcattt ggtgaccccg acgtgatagt tagggaatag
     9301 tggtcggcca cagacggcgt ggcgatcctg tctccatccg tctcgtctat cgggaggcga
     9361 gttcgatgac cctggtggag ggggctgcgg cttagggagg cagaagctga gtaccgtcgg
     9421 aggggatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta
     9481 gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg
     9541 cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc
     9601 tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc
     9661 ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac
     9721 acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttccaaa
     9781 cttggctgtt tcctgtgtga aattgttatc cgctcacaat tccacacatt atacgagccg
     9841 gaagcataaa gtgtaaaacc tggggtgcct aatgagtgag aattcttgaa gacgaaaggg
     9901 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     9961 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    10021 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    10081 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    10141 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    10201 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
    10261 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    10321 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    10381 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    10441 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    10501 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    10561 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    10621 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    10681 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    10741 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    10801 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    10861 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    10921 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10981 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    11041 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    11101 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    11161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    11221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
    11281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    11341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    11401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    11461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    11521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    11581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    11641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    11701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    11761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    11821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    11881 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
    11941 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
    12001 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
    12061 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
    12121 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
    12181 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
    12241 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
    12301 ttaagggcgg ttttttcctg tttggtcact tgatgcctcc gtgtaagggg gaatttctgt
    12361 tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg
    12421 atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc
    12481 gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg
    12541 ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg
    12601 ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg
    12661 ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt
    12721 cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca
    12781 cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc
    12841 tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc
    12901 tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc
    12961 ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca
    13021 gacaaggtat agggcggcga tgcgatgtac gggccagata tacgcgtatc tgaggggact
    13081 agggtgtgtt taggcgaaaa gcggggcttc ggttgtacgc ggttaggagt ccccttagga
    13141 tatagtagtt tcgcttttgc atagggaggg ggaaatgtag tcttatgcaa tactcttgta
    13201 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
    13261 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
    13321 gggtctgaca tggattggac gaaccactga attccgcatt gcagagatat tgtatttaag
    13381 tgcctagctc gatacaataa ac
//
