LOCUS       sequence-69642-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled 
            Inducible Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Bryan EM, Bae T, Kleerebezem M, Dunny GM
  TITLE     Improved vectors for nisin-controlled expression in gram-positive 
            bacteria.
  JOURNAL   Plasmid. 2000 Sep;44(2):183-90.
  PUBMED    10964628
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2000-09"; volume: "44(2)"; pages: "183-90"
COMMENT     Sequence Label: pMSP3535
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cttttgcaac caactgttta aggctaaaag gcttagtaat atagtcatcc ccaccaatac
       61 ctaagccgtt tataatatcc tcttctgtat ctttcgcact aacaaagata attggagttg
      121 atatttctct gcgaatcctt ttacaaattt ctgtcccttc aatatttgac atcatgatat
      181 ctaacaaaat caaatcaaat ccctgaaaat cagtaatatc caagggaagt gaaatgtttt
      241 gatgcgtcgc aacttcatag tttctcattt ctaatgctgt cttcattaat tttaaaattt
      301 cctgatcatc atcaactatt aaaattttat acaccacttt acctccgatt gtttagtact
      361 cattacttca ttataccatt cattctgaag aaatataatc tatcaatttt tagtctttct
      421 tttcaatata ccaaggattg agatacacac tatacctaaa gccgggaatt ctcatgtttg
      481 acagcttatc atcggagctc cgatgataag ctgtcaaaca tgagaattct atttaatcac
      541 tttgactagc aaatactaac aacaagacac acacaccaaa aatcaaaaat tcactacttt
      601 tagttaaaaa ccacgtaacc acaagaacta atccaatcca tgtaatcggg ttcttcaaat
      661 atttctccaa gattttcctc ctctaatatg ctcaacttaa atgacctatt caataaatct
      721 attatgctgc taaatagttt ataggacaaa taagtatact ctaatgacct ataaaagata
      781 gaaaattaaa aaatcaagtg ttcgcttcgc tctcactgcc cctcgacgtt ttagtagcct
      841 ttccctcact tcgttcagtc caagccaact aaaagtttcc gggctactct ctccttctcc
      901 ccctaataat taattaaaat cttactctgt atatttctgc taatcattcg ctaaacagca
      961 aagaaaaaac aaacacgtat catagatata aatgtaatgg catagtgcgg gttttatttt
     1021 cagcctgtat catagctaaa caaatcgagt
//
