LOCUS       sequence-69645-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  in vitro transcription and translation of HA-tagged Notch3 
            Intracellular domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Lardelli M, Williams R, Mitsiadis T, Lendahl U
  TITLE     Expression of the Notch 3 intracellular domain in mouse central 
            nervous system progenitor cells is lethal and leads to disturbed 
            neural tube development.
  JOURNAL   Mech Dev. 1996 Oct;59(2):177-90.
  PUBMED    8951795
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mech Dev.";
            date: "1996-10"; volume: "59(2)"; pages: "177-90"
COMMENT     Sequence Label: CMV/N3IC-HA
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctgtac cagatatcag gatcggtacc gggccccccc tcgacaccac catggttgcc
      121 aggcgaaagc gagaacacag caccctctgg ttccctgagg gttttgcatt acacaaggac
      181 atagctgctg gccacaaggg ccggagggag cctgtgggac aagatgcact gggaatgaag
      241 aacatggcca agggtgagag tctgatgggg gaggtggtca cagacttgaa tgactcagaa
      301 tgtccagagg ccaagagact gaaggtagag gagccgggca tgggtgcaga ggagcctgag
      361 gactgtcgcc agtggaccca acaccacctg gttgctgctg atatccgtgt ggcaccagcc
      421 acagcgctga ctcctcctca gggagatgca gatgcagacg gagtggatgt caacgtccga
      481 gggcctgatg gcttcacccc acttatgctg gcctccttct gtgggggagc cctggagccg
      541 atgccagctg aggaggatga ggcggatgac acatcagcca gcattatctc agatctgatc
      601 tgtcaagggg cccagctcgg ggcacggact gaccgcactg gcgagaccgc cctgcatttg
      661 gctgcccgct atgctagagc ggatgcagcc aagcgtctcc tggatgctgg ggcggacacc
      721 aatgcccagg atcattcggg ccgcacccct ctgcacaccg cagtgacagc tgatgcccag
      781 ggtgtcttcc agattctcat caggaaccgc tccactgacc tggatgcccg aatggcagat
      841 ggctctactg cactgatcct ggcagcccgc ctgg
//
