LOCUS       sequence-69647-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  in vitro transcription and translation of HA-tagged Notch3 
            Intracellular domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Lardelli M, Williams R, Mitsiadis T, Lendahl U
  TITLE     Expression of the Notch 3 intracellular domain in mouse central 
            nervous system progenitor cells is lethal and leads to disturbed 
            neural tube development.
  JOURNAL   Mech Dev. 1996 Oct;59(2):177-90.
  PUBMED    8951795
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mech Dev.";
            date: "1996-10"; volume: "59(2)"; pages: "177-90"
COMMENT     Sequence Label: CMV/N3IC-HA
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(271..291)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          421..486
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(586..612)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag tcaaggcact
      121 atacatcaaa tatttttcca taattttctt gtatagcagt gcagcttttt cctttgtggt
      181 gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca aaaaacttag
      241 caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg gaggagtaga
      301 atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg agcaaaacag
      361 gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat aggttggaat
      421 ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta aaaattttat
      481 atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca caccacagaa
      541 gtaaggttcc ttcacaaaga tcctctagct acctagctag atctatgcat agtctggtac
      601 gtcatatgga taggccatca cctgcctctt gggggtaact tctggttggg gtcccagctg
      661 agtctgggac tgagggaggg agggaacaga tatggggtgt ggctgagcag gcagggctcc
      721 agaggctgtg gcattggtag cagttgctgg gctaggtgtt gagtcagacc agtctgagag
      781 ggatgggggg gatgggctgg cccagtgctc tggggactca ggagacgggg tcaaataagg
      841 atgctcgctg ggaa
//
