LOCUS       sequence-6965-ex       13398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Loftus SK, Larson DM, Watkins-Chow D, Church DM, Pavan WJ
  TITLE     Generation of RCAS vectors useful for functional genomic analyses.
  JOURNAL   DNA Res. 2001 Oct 31. 8(5):221-6.
  PUBMED    11759842
REFERENCE   2  (bases 1 to 13398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Res. 
            2001 Oct 31. 8(5):221-6."
COMMENT     Sequence Label: RCASBP-Y CHA
FEATURES             Location/Qualifiers
     source          1..13398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    7048..7172
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7197..7202,7203..7220,7221..7227)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(7347..7366)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     8463..8482
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8625..8749)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             8760..8786
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        8936..9197
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(9788..9810)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9799..9815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9823..9829,9830..9847,9848..9853))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(9896..9914)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9982..10086
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10087..10155,10156..10947)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10305..10324)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11118..11706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11607..11626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11860..11877
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11892..12032
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11958..11977)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12118..12140
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(12134..12325)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        join(13170..13398,1..33)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gccatttgac cattcaccac attggtgtgc acctgggttg atggccggac cgttgattcc
       61 ctgacgacta cgagcacctg catgaagcag aaggcttcat ttggtgaccc cgacgtgata
      121 gttagggaat agtggtcggc cacagacggc gtggcgatcc tgtctccatc cgtctcgtct
      181 atcgggaggc gagttcgatg accctggtgg agggggctgc ggcttaggga ggcagaagct
      241 gagtaccgtc ggagggagct ccagggcccg gagcgactga cccctgccga gaactcagag
      301 ggtcgtcgga agacggagag tgagcccgac gaccacccca ggcacgtctt tggtcggcct
      361 gcggatcaag catggaagcc gtcattaagg tgatttcgtc cgcgtgtaaa acctattgcg
      421 ggaaaatctc tccttctaag aaggaaatag gggccatgtt gtccctgtta caaaaggaag
      481 ggttgcttat gtctccctca gatttatatt ctccggggtc ctgggatccc atcactgcgg
      541 cgctctccca gcgggcaatg gtacttggaa aatcgggaga gttaaaaacc tggggattgg
      601 ttttgggggc attgaaggcg gctcgagagg aacaggttac atctgagcaa gcaaagtttt
      661 ggttgggatt agggggaggg agggtctctc ccccaggtcc ggagtgcatc gagaaaccag
      721 ctacggagcg gcgaatcgac aaaggggagg aggtgggaga aacaactgtg cagcgagatg
      781 cgaagatggc gccagaggaa gcggccacac ctaaaaccgt tggcacatcc tgctatcatt
      841 gcggaacagc tgttggctgc aattgcgcca ccgccacagc ctcggcccct cctccccctt
      901 atgtggggag tggtttgtat ccttccctgg cgggggtggg agagcagcag ggccagggag
      961 ataacacgtc tcggggggcg gagcagccaa gggaggagcc agggcacgcg ggtcaggccc
     1021 ctgggccggc cctgactgac tgggcaaggg taagggagga gcttgcgagt actggtccgc
     1081 ccgtggtggc catgcctgta gtgattaaga cagagggacc cgcctggacc cctctggagc
     1141 caaaattgat cacaagactg gctgatacgg tcaggaccaa gggcttacga tccccgatca
     1201 ctatggcaga agtggaagcg ctcatgtcct ccccgttgct gccgcatgac gtcacgaatc
     1261 taatgagagt gattttagga cctgccccat atgccttatg gatggacgct tggggagtcc
     1321 aactccagac ggttatagcg gcagccactc gcgacccccg acacccagcg aacggtcaag
     1381 ggcgggggga acggactaac ttggatcgat taaagggctt agctgatggg atggtgggca
     1441 acccacaggg tcaggccgca ttattaagac cgggggaatt ggttgctatt acggcgtcgg
     1501 ctctccaggc gtttagagaa gttgcccggc tggcggaacc tgcaggtcca tgggcggaca
     1561 tcacgcaggg accatctgag tcctttgttg attttgccaa tcggcttata aaggcggttg
     1621 aggggtcaga tctcccgcct tccgcgcggg ctccggtgat cattgactgc tttaggcaga
     1681 agtcacagcc agatattcag cagcttatac gggcagcacc ctccacgctg accaccccag
     1741 gagagataat caaatatgtg ctagacaggc agaagattgc ccctcttacg gatcaaggca
     1801 tagccgcggc catgtcgtct gctatccagc ccttagttat ggcagtagtc aatagagaga
     1861 gggatggaca aactgggtcg ggtggtcgtg cccgagggct ctgctacact tgtggatccc
     1921 cgggacatta tcaggcacag tgcccgaaaa aacgaaagtc aggaaacagc cgtgagcgat
     1981 gtcagctgtg tgacgggatg ggacacaacg ctaaacagtg taggaagcgg gatggcaacc
     2041 agggccaacg cccaggaaga ggtctctctt cggggccgtg gcccggccct gagcagcctg
     2101 ccgtctcgtt agcgatgaca atggaacata aagatcgccc cttggttagg gtcattctga
     2161 ctaacactgg gagtcatcca gtcaaacaac gttcggtgta tatcaccgcg ctgttggact
     2221 ccggagcgga catcactatt atttcggagg aggattggcc tactgattgg ccggtggtgg
     2281 acaccgcgaa cccacagatc catggcatag gagggggaat tcccatgcga aaatcccggg
     2341 atatgataga ggtgggggtt attaaccgag acgggtcgtt ggagcgaccc ctgctcctct
     2401 tccccgcagt cgctatggtt agagggagta tcctaggaag agattgtctg cagggcctag
     2461 ggctccgctt gacaaattta tagggagggc cactgttctt actgttgcgc tacatctggc
     2521 tattccgctc aaatggaagt cagaccgcac gcctgtgtgg attgaccagt ggccccttcc
     2581 tgaaggtaaa cttgtagcgc taacgcaatt agtggaaaaa gaattacagt taggacatat
     2641 agagccctca cttagttgtt ggaacacacc tgtttttcgt gatccggaag gcttccgggt
     2701 cttatcgctt attgcatgat ttgcgcgctg ttaacgccaa gcttgtccct tttggggccg
     2761 tccaacaggg ggcgccagtt ctctccgcgc tcccgcgtgg ctggcccctg atggtcctag
     2821 acctcaagga ttgcttcttt tctatccctc ttgcggaaca agatcgcgaa gcttttgcat
     2881 ttacgctccc ctctgtgaat aaccaggccc ccgctcgaag attccaatgg aaggtcttgc
     2941 cccaagggat gacctgttct cccactatct gtcagttggt agtgggtcag gtgctcgagc
     3001 ccttgcgact caagcaccca gctctgcgca tgttgcatta tatggacgat cttttgctag
     3061 ccgcctcaag tcatgatggg ttggaagcgg cagggaagga ggttatcggt acattggaaa
     3121 gagccgggtt cactatttcg ccggataaga tccagaggga gcccggagta caatatcttg
     3181 ggtacaagtt aggcagtacg tatgtagcac ccgtaggctt ggtagcagaa cccaggatag
     3241 ccaccttgtg ggatgttcaa aagctggtgg ggtcacttca gtggcttcgc ccagcgttag
     3301 ggatcccgcc acgactgatg ggtccctttt atgagcagtt acgagggtca gatcctaacg
     3361 aggcgaggga atggaatcta gacatgaaaa tggcctggag agagatcgta cagcttagca
     3421 ctactgctgc cttggaacga tgggaccctg cccagcctct ggaaggagcg gtcgctagat
     3481 gtgaacaggg ggcaataggg gtcctgggac agggactgtc cacacaccca aggccatgtt
     3541 tgtggttatt ctccacccaa cccaccaagg cgtttactgc ttggttagaa gtgctcaccc
     3601 ttttgattac taagctacgc gcttcggcag tgcgaacctt tggcaaggag gttgatatcc
     3661 tcctgttgcc tgcatgcttc cgggaggacc ttccgctccc ggaggggatc ctgttagcac
     3721 ttagggggtt tgcaggaaaa atcaggagta gtgacacgcc atctattttt gacattgcgc
     3781 gtccactgca tgtttctctg aaagtgaggg ttaccgacca ccctgtgccg ggacccactg
     3841 tctttaccga cgcctcctca agcacccata aaggggtggt agtctggagg gagggcccaa
     3901 ggtgggagat aaaagaaata gttgatttgg gggcaagtgt acaacaactg gaggcacgcg
     3961 ctgtggccat ggcacttctg ctgtggccga caacgcccac taatgtagtg actgactctg
     4021 cgtttgttgc gaaaatgtta ctcaagatgg gacaggaggg agtcccgtct acagcggcag
     4081 cttttatttt agaggatgcg ttaagccaaa ggtcagccat ggccgccgtt ctccacgtgc
     4141 ggagtcattc tgaagtgcca gggtttttca cagaaggaaa tgacgtggca gatagccaag
     4201 ccacctttca agcgtatccc ttgagagagg ctaaagatct tcataccgct ctccatattg
     4261 gaccccgcgc gctatccaaa gcgtgtaata tatctatgca gcaggctagg gaggttgttc
     4321 agacctgccc gcattgtaat tcagcccctg cgttggaggc cggggtaaac cctaggggtt
     4381 tgggacccct acagatatgg cagacagact ttacgcttga gcctagaatg gctccccgtt
     4441 cctggctcgc tgttactgtg gacaccgcct catcagcgat agtcgtaact cagcatggcc
     4501 gtgttacatc ggttgctgca caacatcatt gggccacggc tatcgccgtt ttgggaagac
     4561 caaaggccat aaaaacagat aacgggtcct gcttcacgtc tagatccacg cgggagtggc
     4621 tcgcgagatg ggggatagca cacaccaccg ggattccggg aaattcccag ggtcaagcta
     4681 tggtagagcg ggccaaccgg ctcctgaaag ataagatccg tgtgcttgcg gagggggacg
     4741 gctttatgaa aagaatcccc gccagcaaac agggggaact actagccaag gcaatgtatg
     4801 ccctcaatca ctttgagcgt ggtgaaaaca caaaaacacc ggtacaaaaa cactggagac
     4861 ctaccgttct tacagaagga cccccggtta aaatacgaat agagacaggg gagtgggaaa
     4921 aaggatggaa cgtgctggtc tggggacgag gttatgccgc tgtgaaaaac agggacactg
     4981 ataaggttat ttgggtaccc tctcggaaag ttaaaccgga tgtcacccaa aaggatgagg
     5041 tgactaagaa agatgaggcg agccctcttt ttgcaggcat ttctgactgg ataccctggg
     5101 aagacgagca agaaggactc caaggagaaa ccgctagcaa caagcaagaa agacccggag
     5161 aagacaccct tgctgccaac gagagttaat tatattctca ttattggtgt cctggtcttg
     5221 tgtgaggtta cgggggtaag agctgatgtc cacttactcg agcagccagg gaacctttgg
     5281 attacatggg ccaaccgtac aggccaaacg gatttttgcc tctctacaca gtcagccacc
     5341 tccccttttc aaacatgttt gataggtatc ccgtccccta tttccgaggg tgattttaag
     5401 ggatatgttt ctgatacaaa ttgcaccacc ttgggaactg atcggttagt ctcgtcagcc
     5461 gactttactg gcggacctga caacagtacc accctcactt atcggaaggt ctcatgcttg
     5521 ttgttaaagc tgaatgtctc tatgtgggat gagccacctg aactacagct gttaggttcc
     5581 cagtctctcc ctaacattac taatattgct cagatttccg gtataaccgg gggatgcgta
     5641 ggcttcagac cacaaggggt tccttggtat ctaggttggt ctagacagga ggccacgcgg
     5701 tttctcctta gacacccctc tttctctaaa tccacggaac cgtttacagt ggtgacagcg
     5761 gataggcaca atctttttat ggggagtgag tactgcggtg catatggcta cagattttgg
     5821 aacatgtata actgctcaca ggtggggcgg cagtaccgct gtggtaatgc gcgcacgccc
     5881 cgcacgggtc ttcctgaaat ccagtgtaca aggagaggag gcaaatgggt taatcaatca
     5941 caggaaatta atgagtcgga gccgttcagc tttacggtga actgtacagc tagtagtttg
     6001 ggtaatgcca gtgggtgttg cggaaaagca ggcacgattc tcccgggaaa gtgggtcgac
     6061 agcacacaag gtagtttcac caaaccaaaa gcgctaccac ccgcaatttt cctcatttgt
     6121 ggggatcgcg catggcaagg aattcccagt cgtccggtag ggggcccctg ctatttaggc
     6181 aagcttacca tgttagcacc taagcataca gatattctca aggtgcttgt caattcatcg
     6241 cggacaggta taagacgtaa acgaagcacc tcacacctgg atgatacatg ctcagatgaa
     6301 gtgcagcttt ggggtcctac agcaagaatc tttgcatcta tcctagcccc gggggtagca
     6361 gctgcgcaag ccttaagaga aattgagaga ctagcctgtt ggtccgttaa acaggctaac
     6421 ttgacaacat cactcctcgg ggacttattg gatgatgtca cgagtattcg acacgcggtc
     6481 ctgcagaacc gagcggctat tgacttcttg ctcctagctc acggccatgg ctgtgaggac
     6541 gttgccggaa tgtgctgttt caatttgagt gatcagagtg agtctataca gaagaagttc
     6601 cagctaatga aggaacatgt caataagatc ggcgtggata gcgacctaat tggaagttgg
     6661 ctgcgaggac tattcggggg aataggagaa tgggccgttc atttgctgaa aggactgctt
     6721 ttggggcttg tagttatttt gttgctagta gtgtgcctgc cttgcctttt gcaaatgtta
     6781 tgcggtaata ggagaaagat gattaataac tccatcagct accacacgga atataagaag
     6841 ctgcaaaagg cctgtgggca gcctgaaagc agaatagtat aaggcagtac atgggtggtg
     6901 gtatagcgct tgcgagtcca tcgagcaagg caggaaagac agctattggt aattgtgaaa
     6961 tacgcttttg tctgtgtgct gcaggagctg agctgactct gctggtggcc tcgcgtacca
     7021 ctgtggcatc ggcggccgcg tttatcaaca agtttgtaca aaaaagctga acgagaaacg
     7081 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     7141 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
     7201 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
     7261 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     7321 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     7381 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     7441 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     7501 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     7561 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     7621 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     7681 tccctaaggg tttattgaga atatgttttt cgtctcagcc aatcctgggt gagtttcacc
     7741 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     7801 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     7861 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     7921 tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg
     7981 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     8041 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     8101 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     8161 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     8221 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     8281 tgcagtttaa ggtttacacc tataaaagag agagcgttat cgtctgtttg tggatgtaca
     8341 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     8401 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     8461 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     8521 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     8581 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
     8641 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     8701 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg ataaactcct
     8761 acccttatga cgtgccagat tatgcctgaa tttaaatatc gatgcgatgt acgggccaga
     8821 tatacgcgta tctgagggga ctagggtgtg tttaggcgaa aagcggggct tcggttgtac
     8881 gcggttagga gtccccttag gatatagtag tttcgctttt gcatagggag ggggaaatgt
     8941 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     9001 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     9061 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattccgca
     9121 ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgccatt tgaccattca
     9181 ccacattggt gtgcacctgg gttgatggcc ggaccgttga ttccctgacg actacgagca
     9241 cctgcatgaa gcagaaggct tcatttggtg accccgacgt gatagttagg gaatagtggt
     9301 cggccacaga cggcgtggcg atcctgtctc catccgtctc gtctatcggg aggcgagttc
     9361 gatgaccctg gtggaggggg ctgcggctta gggaggcaga agctgagtac cgtcggaggg
     9421 gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     9481 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     9541 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
     9601 ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
     9661 agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg
     9721 tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tccaaacttg
     9781 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacattatac gagccggaag
     9841 cataaagtgt aaaacctggg gtgcctaatg agtgagaatt cttgaagacg aaagggcctc
     9901 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     9961 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    10021 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    10081 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    10141 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    10201 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    10261 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    10321 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    10381 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    10441 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    10501 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    10561 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    10621 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    10681 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    10741 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    10801 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    10861 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    10921 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    10981 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    11041 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    11161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    11221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
    11281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    11341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    11401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    11461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    11521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    11581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    11641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    11701 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    11761 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    11821 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    11881 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
    11941 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
    12001 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
    12061 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    12121 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
    12181 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
    12241 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
    12301 gggcggtttt ttcctgtttg gtcacttgat gcctccgtgt aagggggaat ttctgttcat
    12361 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
    12421 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
    12481 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
    12541 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
    12601 cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca
    12661 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
    12721 ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
    12781 catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga
    12841 gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg
    12901 caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct
    12961 tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca
    13021 aggtataggg cggcgatgcg atgtacgggc cagatatacg cgtatctgag gggactaggg
    13081 tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc ttaggatata
    13141 gtagtttcgc ttttgcatag ggagggggaa atgtagtctt atgcaatact cttgtagtct
    13201 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
    13261 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
    13321 ctgacatgga ttggacgaac cactgaattc cgcattgcag agatattgta tttaagtgcc
    13381 tagctcgata caataaac
//
