LOCUS       sequence-69651-e         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains PBAD::GFP reporter, used for heterodimer competition assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Su PC, Berger BW
  TITLE     A Novel Assay for Assessing Juxtamembrane and Transmembrane Domain 
            Interactions Important for Receptor Heterodimerization.
  JOURNAL   J Mol Biol. 2013 Jul 19. pii: S0022-2836(13)00456-7. doi: 
            10.1016/j.jmb.2013.07.022.
  PUBMED    23876708
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2013 Jul 19. pii: S0022-2836(13)00456-7. doi: 
            10.1016/j.jmb.2013.07.022."
COMMENT     Sequence Label: pAraGFPCDF
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    169..193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(193..212)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(194..212)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(345..365)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agggtaccga cgtcagcgat cgcgtggccg gccgatatcc aattgagatc
      121 tgccatatgt atatctcctt cttatactta actaatatac taagatgggg aattgttatc
      181 cgctcacaat tcccctatag tgagtcgtat taatttcgat tatgcggccg tgtacaatac
      241 gattactttc tgttcgactt aagcattatg cggccgcaag cttgtcgacc tgcagaaaaa
      301 attatttgta tagttcatcc atgccatgtg taatcccagc agctgttaca aactcaagaa
      361 ggaccatgtg gtctctcttt tcgttgggat ctttcgaaag ggcagattgt gtggacaggt
      421 aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt tgataatggt
      481 ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat tttgaagtta actttgattc
      541 cattcttttg tttgtctgcc atgatgtata cattgtgtga gttatagttg tattccaatt
      601 tgtgtccaag aatgtttcca tcttctttaa aatcaatacc ttttaactcg attctattaa
      661 caagggtatc accttcaaac ttgacttcag cacgtgtctt gtagttcccg tcatctttga
      721 aaaatatagt tctttcctgt acataacctt cgggcatggc actcttgaaa aagtcatgct
      781 gtttcatatg atctgggtat ctcgcaaagc attgaagacc atacgcgaaa gtagtgacaa
      841 gtgttggcca tggaacaggt agttttccag tagtgcaaat aaatttaagg gtaagttttc
      901 cgtatgttgc atcaccttca ccctctccac tgacagaaaa tttgtgccca ttaacatcac
      961 catctaattc aacaagaatt gggac
//
