LOCUS       sequence-69652-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains PBAD::GFP reporter, used for heterodimer competition assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Su PC, Berger BW
  TITLE     A Novel Assay for Assessing Juxtamembrane and Transmembrane Domain 
            Interactions Important for Receptor Heterodimerization.
  JOURNAL   J Mol Biol. 2013 Jul 19. pii: S0022-2836(13)00456-7. doi: 
            10.1016/j.jmb.2013.07.022.
  PUBMED    23876708
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2013 Jul 19. pii: S0022-2836(13)00456-7. doi: 
            10.1016/j.jmb.2013.07.022."
COMMENT     Sequence Label: pAraGFPCDF
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     112..131
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        112..130
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    131..155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             211..228
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        349..633
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     556..575
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(724..752)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
       61 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaaa ttaatacgac
      121 tcactatagg ggaattgtga gcggataaca attcccctgt agaaataatt ttgtttaact
      181 ttaataagga gatataccat gggcagcagc catcaccatc atcaccacag ccaggatccg
      241 aattcatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
      301 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
      361 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaacca
      421 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
      481 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
      541 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
      601 gctttttatc gcaactctct actgtttctc catacccgtt tttttgggaa aaaaaaacgg
      661 ggatcctcta gatttaatga aggagatata catatgagta aaggagaaga acttttcact
      721 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      781 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      841 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcgcgta tggtcttcaa
      901 tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccg
      961 a
//
