LOCUS       sequence-69653-e        1083 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codes for the Active/Repressed TLR with the target site for I-Sce I 
            nuclease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.Active/Repressed TLR (Sce)
FEATURES             Location/Qualifiers
     source          1..1083
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             73..1020
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     CDS             1027..1080
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cccctcactc ggcgcgccag tcctccgaca gactgagtcg atcgcttcag tcaagattta
       61 attaacgcca ccatggctga aggatccgtc gccaggcagc ctgacctctt gacctgcgac
      121 gatgagccga tccatatccc cggtgccatc caaccgcatg gactgctgct cgccctcgcc
      181 gccgacatga cgatcgttgc cggcagcgac aaccttcccg aactcaccgg actggcgatc
      241 ggcgccctga tcggccgctc tgcggccgat gtcttcgact cggagacgca caaccgtctg
      301 acgatcgcct tggccgagcc cggggcggcc gtcggagcac cgatcactgt cggcttcacg
      361 atgcgaaagg acgcaggctt catcggctcc tggcatcgcc atgatcagct catcttcctc
      421 gagctcgagc ctccccagcg ggacgtcgcc gagccgcagg cgttcttccg ccgcaccaac
      481 agcgccatcc gccgcctgca ggccgccgaa accttggaaa gcgcctgcgc cgccgcggcg
      541 caagaggtgc ggaagattac cggcttcgat cgggtgatga tctatcgctt cgcctccgac
      601 ttcagcggcg aagtgatcgc agaggatcgg tgcgccgagg tcgagtcaaa actaggcctg
      661 cactatcctg cctcaaccgt gccggcgcag gcccgtcggc tctataccat caacccggta
      721 cggatcattc ccgatatcaa ttatcggccg gtgccggtca ccccagacct caatccggtc
      781 accgggcggc cgattgatct tagcttcgcc atcctgcgca gcgtctcgcc cgtccatctg
      841 gaattcatgc gcaacatagg catgcacggc acgatgtcga tctcgatttt gcgcggcgag
      901 cgactgtggg gattgatcgt ttgccatcac cgaacgccgt actacgtcga tctcgatggc
      961 cgccaagcct gcgagctagt cgcccaggtt ctggcctggc agatcggcgt gatggaagag
     1021 tctagagagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tccgggcccc
     1081 gtg
//
