LOCUS       sequence-69654-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codes for the Active/Repressed TLR with the target site for I-Sce I 
            nuclease.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.Active/Repressed TLR (Sce)
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..759)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(330..349)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     599..617
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(760..813)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(990..1011)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt gactaatgca ttcacttgta
       61 cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact gttccacgat
      121 ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt aggcgccggg
      181 cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc
      241 gtccttcagc ttcagcctct gcttgatctc gcccttcagg gcgccgtcct cggggtacat
      301 ccgctcggag gaggcctccc agcccatggt cttcttctgc attacggggc cgtcggaggg
      361 gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gagaggagtc
      421 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
      481 ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt
      541 ggagccgtac atgaactgag gggacaggat gtcccaggcg aagggcaggg ggccaccctt
      601 ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc
      661 gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcaggaactc
      721 cttgatgatg gcggagttat cctcctcgcc cttgctcaca gggcccggat tctcctccac
      781 gtcaccgcat gttagaagac ttcctctgcc ctcacccacg ttgatcctag cgctggcgca
      841 agcggcgggg tgcctgtcca tgccgctctc ctgggcgcag ctcatgggca gggtgccatc
      901 gtcctgagcg gccacctcag gggggaagcc gtggctggcc ggccttactt gtacagctcg
      961 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     1021 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggt
//
