LOCUS       sequence-69655-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Notch reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Hansson EM, Teixeira AI, Gustafsson MV, Dohda T, Chapman G, Meletis 
            K, Muhr J, Lendahl U
  TITLE     Recording Notch signaling in real time.
  JOURNAL   Dev Neurosci. 2006;28(1-2):118-27.
  PUBMED    16508309
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev 
            Neurosci."; date: "2006"; volume: "28(1-2)"; pages: "118-27"
COMMENT     Sequence Label: 12XCSL-d1EGFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    253..374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(290..309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     344..363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(403..419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(403..419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(416..438)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    427..443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(451..457,458..475,476..481))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    496..517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(634..651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agaagcttag ccatggcttc ccgccggcgg tggcggcgca ggatgatggc acgctgccca
       61 tgtcttgtgc ccaggagagc gggatggacc gtcaccctgc agcctgtgct tctgctagga
      121 tcaatgtgta ggcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
      181 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      241 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      301 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
      361 atcaatgtat cttaagggta ccgagctcga attcgtaatc atgtcatagc tgtttcctgt
      421 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      481 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      541 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      601 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      661 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      721 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      781 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      841 aaatcgacgc tcaagtcaga
//
