LOCUS       sequence-69658-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used to create transgenic mice expressing LacZ reporter with Nestin 
            enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Lothian C, Lendahl U
  TITLE     An evolutionarily conserved region in the second intron of the human
            nestin gene directs gene expression to CNS progenitor cells and to 
            early neural crest cells.
  JOURNAL   Eur J Neurosci. 1997 Mar;9(3):452-62.
  PUBMED    9104587
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Neurosci."; date: "1997-03"; volume: "9(3)"; pages: "452-62"
COMMENT     Sequence Label: nes1852tk/lacZ
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..162
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     intron          365..461
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        complement(518..580)
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
ORIGIN
        1 aaccaggcaa agcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
       61 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
      121 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacgggatc gcgcttgagc
      181 agctccttgc tggtgtccag accaatgcct cccagaccgg caacgaaaat cacgttcttg
      241 ttggtcaaag taaacgacat ggtgacttct tttttgcttt agcaggctct ttcgatcccc
      301 gggaattgcg gccgcgggta caattccgca gcttttagag cagaagtaac acttccgtac
      361 aggcctagaa gtaaaggcaa catccactga ggagcagttc tttgatttgc accaccaccg
      421 gatccgggac ctgaaataaa agacaaaaag actaaactta ccagttaact ttctggtttt
      481 tcagttcctc gagatctgcg gcacgctgtt gacgctgtta agcgggtcgc tgcagggtcg
      541 ctcggtgttc gaggccacac gcgtcacctt aatatgcgaa gtggacctcg gaccgcgccg
      601 ccccgactgc atctgcgtgt tcgaattcgc caatgacaag acgctgggcg gggccggatc
      661 ctctagagtc gacaagtctt ggagccaccg ccaggtgttt gcagccggga gttctcagcc
      721 tccaggaggg tcctggagag gacagaggga aagtggggtc agccctctgc cccca
//
