LOCUS       sequence-69661-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used to create transgenic mice expressing LacZ reporter with Nestin 
            enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Lothian C, Lendahl U
  TITLE     An evolutionarily conserved region in the second intron of the human
            nestin gene directs gene expression to CNS progenitor cells and to 
            early neural crest cells.
  JOURNAL   Eur J Neurosci. 1997 Mar;9(3):452-62.
  PUBMED    9104587
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Neurosci."; date: "1997-03"; volume: "9(3)"; pages: "452-62"
COMMENT     Sequence Label: nes1852tk/lacZ
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     171..193
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     185..202
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     186..202
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
       61 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      121 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      181 acgttgtaaa acgacggcca gtgccaagct tgcatgcctg caggtcgacg gagcaggaga
      241 aacagggcct acagagccag atcgctcagg tcctggaagg tcggcagcag ctggcgcacc
      301 tcaagatgtc cctcagcctg gaggtggcca cgtacaggta cgagacgagc cctgtccacc
      361 caggcacaca gaggcacaca cacatgctgg ggcagactac gtggctgaag gggaccagag
      421 gtgtaggtga tggcaactct gctgccactg ggttctgtga ctctaggctg gtcacttcca
      481 caccggaaca tgtcctgcct gaaaccgtct gctcccccat aggctggaga cctgagcaat
      541 gtcctgtgcc caggaaatcc ccccaggaga gatgatattg ccgagcggga cagcctctgg
      601 agaggggctg ggcccgtgtg gggcaactgc atccagaccc ttcccccaag gggcaaagct
      661 ccaactctat gtgtgcccgc acccaggggg tcatgtcctt tggcagccac ggaaaggagg
      721 cattgcaggc atggggtggc tgcactgact tgccacctga cctgggcctc agcctcgtct
      781 aggggctgag ggctgggctg ggggacttcc aaggcctctt ctagcctgga tgtcatgggg
      841 cgagactcct ctgatgcccc tagtgccctt ggtcctgccc agcctttcat tgtccacatt
      901 ttctgttatc cta
//
