LOCUS       sequence-69663-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codes for the SSA-TLR with the target site for I-Ani I in a 
            lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.SSA TLR (Ani)
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             901..954
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(997..1018)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cccctcactc ggcgcgccag tcctccgaca gactgagtcg cccgctcgag cgccaccatg
       61 gctgaaggat ccgtcgccag gcagcctgac ctcttgacct gcgacgatga gccgatccat
      121 atccccggtg ccatccaacc gcatggactg ctgctcgccc tcgccgccga catgacgatc
      181 gttgccggca gcgacaacct tcccgaactc accggactgg cgatcggcgc cctgatcggc
      241 cgctctgcgg ccgatgtctt cgactcggag acgcacaacc gtctgacgat cgccttggcc
      301 gagcccgggg cggccgtcgg agcaccgatc actgtcggct tcacgatgcg aaaggacgca
      361 ggcttcatcg gctcctggca tcgccatgat cagctcatct tcctcgagct cgagcctccc
      421 cagcgggacg tcgccgagcc gcaggcgttc ttccgccgca ccaacagcgc catccgccgc
      481 ctgcaggccg ccgaaacctt ggaaagcgcc tgcgccgccg cggcgcaaga ggtgcggaag
      541 attaccggct tcgatcgggt gatgatctat cgcttcgcct ccgacttcag cggcgaagtg
      601 atcgcagagg atcggtgcgc cgaggtcgag tcaaaactag gcctgcacta tcctgcctca
      661 accgtgccgg cgcaggcccg tcggctctat accatcaacc cggtacggat cattcccgat
      721 atcaattatc ggccggtgcc ggtcacccca gacctcaatc cggtcaccgg gcggccgatt
      781 gatcttagct tcgccatcct gcgcagcgtc tcgcccgtcc atctggaatt catgcgcaac
      841 ataggcatgc acggcacgat gtcgatctcg attttgcgcg gcgagcgact gtggtctaga
      901 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatccggg ccccgtgagc
      961 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1021 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgcc
//
