LOCUS       sequence-69664-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used to create transgenic mice expressing LacZ reporter with Nestin 
            enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Lothian C, Lendahl U
  TITLE     An evolutionarily conserved region in the second intron of the human
            nestin gene directs gene expression to CNS progenitor cells and to 
            early neural crest cells.
  JOURNAL   Eur J Neurosci. 1997 Mar;9(3):452-62.
  PUBMED    9104587
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Neurosci."; date: "1997-03"; volume: "9(3)"; pages: "452-62"
COMMENT     Sequence Label: nes714tk/lacZ
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     187..209
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     201..218
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     202..218
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
       61 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
      121 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      181 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttgca tgcctgcagg
      241 tcgacggatc cagactgctt agagatcctg agatgagggc cctgaccccc agtgaagcca
      301 ccgaggggaa gggtctggtg ggccccgagg aatttaactg ctattttttg aggcatgaaa
      361 aggatttgga gagggggagc tgaattcatt tgcttttgtc tgtcgctagc tctgggggcc
      421 accggggaag gaggagggag cccatcccat gggaacggcc tgagaattcc cacttcccca
      481 gaatcctctc cttctcagac cctccagatg gtggcttgga caatggcagc aattagcatg
      541 agaatcggtc ccccacacag aggatgaggt cattggcctg gggtgggagg aggaggctag
      601 cctaagcctt gtgtgggtgt gtgtagggca atgtccgact gtgtgggaat ctgcttctat
      661 gtctgagctc tgctgtgtat cccaaataca gtgtgacttt gtgtgaccaa gtgtgtgatg
      721 tctgtgggta tgtgactgtg tgatttcacc ctgagtgtgg cttttgtgtg acagtgtcaa
      781 atgaagagga agcctgcagg gcctggatgg tgcaagctca gcctgagttg tccccagctg
      841 gcttgctgcc tgcaggcctg ggggcaga
//
