LOCUS       sequence-69669-e         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of V5-tagged IL10RA in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Kang BH, Jensen KJ, Hatch JA, Janes KA
  TITLE     Simultaneous profiling of 194 distinct receptor transcripts in human
            cells.
  JOURNAL   Sci Signal. 2013 Aug 6;6(287):rs13. doi: 10.1126/scisignal.2003624.
  PUBMED    23921087
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-08-6"; volume: "6(287):rs13. doi"; pages: " 
            10.1126/scisignal.2003624"
COMMENT     Sequence Label: pLX302 IL10RA-V5 puro
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..107)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    109..133
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgac ttaacgcgcc accggttagc gctagctcat
       61 tactacgtag aatcgagacc gaggagaggg ttagggatag gcttaccaac cactttgtac
      121 aagaaagttg ggcactcact tgactgcagg ctagagatga ggggcagggt gaccaggtct
      181 gagttaaagc tgcccaggag accacctggg gctgccacac agcctagagg ggcaaggtca
      241 tgggcaaagc tccagtcaga tattcccagg tcactgcagc tgctcaaggc caggagtgac
      301 cattcctcag tgggctggtt ccactgtccc gttggggccc ctgaggaagc cagagtcatt
      361 tctagaggat cctgtttcaa atagcccttg gccagggctg gtggatgcaa gcctgcctca
      421 tcaaccaggc atctcccgaa tttggggcca aggccatctg tcaagggcga ttcttcctcc
      481 aggcagcctg tcttggttgc cttctcttca gcacatctgg tctgcctcag gtaaccctgg
      541 aatgccacag cagctgggtc ttcttcccca ggcacctcag gctccggggg actgtggtgg
      601 cccaaggccg agccaccctg tgtgtcccca gcccggccct cagagttttg aactaagtca
      661 atgccactgt catcctggcc cctgctgttg ctccccacct gttgctccca ggtgggccct
      721 gtgctggggc tcaggctggg ctcctgcagg cagatcccgc tgtctgtgct attgctgctg
      781 ccactactgc agctgtcccc cagcacaggg ggctccccgt ttcccagcgt tctgtcagcc
      841 tgggggtgag ggtcagggag gaggaactgg ggctcttcag tctgcaggga tggcttggtg
      901 ctgccaaagc cactgtctgt gctgccgtgc aggtccaagt tcttcagctc tggggaca
//
