LOCUS       sequence-69675-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for co-expression of constitutively 
            active MKK4 and MKK7a1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Bose AK, Janes KA
  TITLE     A high-throughput assay for phosphoprotein-specific phosphatase 
            activity in cellular extracts.
  JOURNAL   Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 
            10.1074/mcp.O112.024059. Epub 2012 Dec 11.
  PUBMED    23233447
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/mcp.O112.024059"; journalName: "Mol Cell Proteomics"; date:
            "2013-03"; volume: "12"; issue: "3"; pages: "797-806"
COMMENT     Sequence Label: pCDFDuet-1 MKK4(EE)-MKK7a1(EE)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg acctacctga agaagggcag atggtgctga ctcaggactc cactagtcct
      121 tggggactcg gtcttcgcca tgacatcctt aaaccaggac gcgacatcca cctcgagtat
      181 ctcatagtgc ttgatgaagc tgtgttcaag tagcttatta tactttggtc tcttcctgtg
      241 atctttagta aggcagtctt tgacaaatga ctggaagtcc cctgagaagc ccatgtgacc
      301 aggcaggagt gggggctctt cctgtaggac tttggtgagg acctcaaagt ccgtcttgca
      361 gttcttatag gggaactgtc ctgttgccag ctccaccagt gagatgccca ggctccacac
      421 atcagctcgg atgtcatagt caggcttggt gggatctgga gggtcgatgc gctcgggagc
      481 catataggca gcacagccag cactccgttc tttggctttt tcgtcaacaa ggcggccact
      541 gatgccaaag tcacagagct tgatctggcc ccgctcatct agcaggatgt tggagggttt
      601 gacatcgcga tggatgacgc catgcttctc cttcagatag tacagtgctt tcacaatcgc
      661 cacagtcatc ttgcccagga ttcgctctgg aatggggccc tgcattcgtt tcttcagctt
      721 ctctgcacat gtgcccatga gctccatggc aataaagacg tctgtgttgg tgatgaaggt
      781 gccaaagcac tgaacgatgt aagggcagtc atggctcttg agtactacat ccaggtccat
      841 caaaatgcgc ttattctctt ccttgttccc
//
