LOCUS       sequence-69677-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for co-expression of constitutively 
            active MKK4 and MKK7a1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Bose AK, Janes KA
  TITLE     A high-throughput assay for phosphoprotein-specific phosphatase 
            activity in cellular extracts.
  JOURNAL   Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 
            10.1074/mcp.O112.024059. Epub 2012 Dec 11.
  PUBMED    23233447
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/mcp.O112.024059"; journalName: "Mol Cell Proteomics"; date:
            "2013-03"; volume: "12"; issue: "3"; pages: "797-806"
COMMENT     Sequence Label: pCDFDuet-1 MKK4(EE)-MKK7a1(EE)
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     119..138
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        119..137
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    138..162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             218..235
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
       61 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaaatta
      121 atacgactca ctatagggga attgtgagcg gataacaatt cccctgtaga aataattttg
      181 tttaacttta ataaggagat ataccatggg cagcagccat caccatcatc accacagcca
      241 ggatccatgc agggtaaacg caaagcactg aagttgaatt ttgcaaatcc acctttcaaa
      301 tctacagcaa ggtttactct gaatcccaat cctacaggag ttcaaaaccc acacatagag
      361 agactgagaa cacacagcat tgagtcatca ggaaaactga agatctcccc tgaacaacac
      421 tgggatttca ctgcagagga cttgaaagac cttggagaaa ttggacgagg agcttatggt
      481 tctgtcaaca aaatggtcca caaaccaagt gggcaaataa tggcagttaa aagaattcgg
      541 tcaacagtgg atgaaaaaga acaaaaacaa cttcttatgg atttggatgt agtaatgcgg
      601 agtagtgatt gcccatacat tgttcagttt tatggtgcac tcttcagaga gggtgactgt
      661 tggatctgta tggaactcat gtctacctcg tttgataagt tttacaaata tgtatatagt
      721 gtattagatg atgttattcc agaagaaatt ttaggcaaaa tcactttagc aactgtgaaa
      781 gcactaaacc acttaaaaga aaacttgaaa attattcaca gagatatcaa accttccaat
      841 attcttctgg aca
//
