LOCUS       sequence-69680-e         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Qiang L, Banks AS, Accili D
  TITLE     Uncoupling of acetylation from phosphorylation regulates FoxO1 
            function independent of its subcellular localization.
  JOURNAL   J Biol Chem. 2010 Aug 27;285(35):27396-401. Epub 2010 Jun 2.
  PUBMED    20519497
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2010-08-27"; volume: "285(35)"; pages: "27396-401. 
            Epub 2010 Jun 2"
COMMENT     Sequence Label: FoxO1-ADA-GFP
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    152..273
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(189..208)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     243..262
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(280..735)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(417..438)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     629..648
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca
       61 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
      121 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
      181 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      241 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
      301 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
      361 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
      421 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
      481 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
      541 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
      601 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
      661 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
      721 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctattt
//
