LOCUS       sequence-69682-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Notch reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Hansson EM, Teixeira AI, Gustafsson MV, Dohda T, Chapman G, Meletis 
            K, Muhr J, Lendahl U
  TITLE     Recording Notch signaling in real time.
  JOURNAL   Dev Neurosci. 2006;28(1-2):118-27.
  PUBMED    16508309
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev 
            Neurosci."; date: "2006"; volume: "28(1-2)"; pages: "118-27"
COMMENT     Sequence Label: 12XCSL-DsRedExpressDL
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    155..207
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(633..650)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(633..649)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(642..664)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(845..863)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc
       61 gaactcgtgg ccgttcacgg agccctccat gcgcaccttg aagcgcatga actccttgat
      121 gacgtcctcg gaggaggcca tggtggcgac cggtggatcc cgggcccgcg gtaccgtcga
      181 ctgcagaatt cgaagcttga gctcgaggga ttgcaagtaa acacagttgt gtcaaaagca
      241 agtgtaagca gaagctgaag tgatctgcct tttatgccca agatgcatac agtggatcta
      301 ctattttccc acggtgccct tccgcccatt ttcccacgag tcgggatcta ctattttccc
      361 acggtgccct tccgcccatt ttcccacgag tcgggatcta ctattttccc acggtgccct
      421 tccgcccatt ttcccatgag tcgggatcta ctattttccc acggtgccct tccgcccatt
      481 ttcccacgag tcgggatcta ctattttccc acggtgccct tccgcccatt ttcccacgag
      541 tcgggatcta ctattttccc acggtgccct tccgcccatt ttcccatgag tcgggatcct
      601 ctagagtcga cctgcaggca tgcaagcttg gcactggccg tcgttttaca acgtcgtgac
      661 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      721 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      781 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
      841 ataggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
      901 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc cta
//
