LOCUS       sequence-6969-ex        8724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8724)
  TITLE     Gateway Destination vectors for E. coli expression
REFERENCE   2  (bases 1 to 8724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDest-544
FEATURES             Location/Qualifiers
     source          1..8724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..41
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        22..40
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    41..65
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             80..102
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             119..136
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             140..1624
                     /codon_start=1
                     /gene="nusA"
                     /product="transcription elongation protein (N utilization 
                     substance protein A)"
                     /label=NusA
                     /note="highly soluble in E. coli"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDV
                     RVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDR
                     ITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVIL
                     REDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIE
                     IKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPA
                     QFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMT
                     VDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLD
                     EPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLED
                     LAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     protein_bind    1634..1758
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1783..1788,1789..1806,1807..1813)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1867..2526
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1933..1952)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2868..3173
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3052..3071
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3214..3338)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3348..3365
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3531..3617
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3709..3736
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3790..3808)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3804..3851
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3888..4343
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3975..3994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4185..4206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(4458..5249,5250..5318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5081..5100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5319..5411)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5492..6080
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5981..6000
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6234..6251
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6266..6408
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6334..6353)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6494..6516
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6510..6701)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7273..8355)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8317..8336
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8356..8433)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     8639..8658
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
       61 attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata tgggatccca
      121 tcaccatcac catcacagta tgaacaaaga aattttggct gtagttgaag ccgtatccaa
      181 tgaaaaggcg ctacctcgcg agaagatttt cgaagcattg gaaagcgcgc tggcgacagc
      241 aacaaagaaa aaatatgaac aagagatcga cgtccgcgta cagatcgatc gcaaaagcgg
      301 tgattttgac actttccgtc gctggttagt tgttgatgaa gtcacccagc cgaccaagga
      361 aatcaccctt gaagccgcac gttatgaaga tgaaagcctg aacctgggcg attacgttga
      421 agatcagatt gagtctgtta cctttgaccg tatcactacc cagacggcaa aacaggttat
      481 cgtgcagaaa gtgcgtgaag ccgaacgtgc gatggtggtt gatcagttcc gtgaacacga
      541 aggtgaaatc atcaccggcg tggtgaaaaa agtaaaccgc gacaacatct ctctggatct
      601 gggcaacaac gctgaagccg tgatcctgcg cgaagatatg ctgccgcgtg aaaacttccg
      661 ccctggcgac cgcgttcgtg gcgtgctcta ttccgttcgc ccggaagcgc gtggcgcgca
      721 actgttcgtc actcgttcca agccggaaat gctgatcgaa ctgttccgta ttgaagtgcc
      781 agaaatcggc gaagaagtga ttgaaattaa agcagcggct cgcgatccgg gttctcgtgc
      841 gaaaatcgcg gtgaaaacca acgataaacg tatcgatccg gtaggtgctt gcgtaggtat
      901 gcgtggcgcg cgtgttcagg cggtgtctac tgaactgggt ggcgagcgta tcgatatcgt
      961 cctgtgggat gataacccgg cgcagttcgt gattaacgca atggcaccgg cagacgttgc
     1021 ttctatcgtg gtggatgaag ataaacacac catggacatc gccgttgaag ccggtaatct
     1081 ggcgcaggcg attggccgta acggtcagaa cgtgcgtctg gcttcgcaac tgagcggttg
     1141 ggaactcaac gtgatgaccg ttgacgacct gcaagctaag catcaggcgg aagcgcacgc
     1201 agcgatcgac accttcacca aatatctcga catcgacgaa gacttcgcga ctgttctggt
     1261 agaagaaggc ttctcgacgc tggaagaatt ggcctatgtg ccgatgaaag agctgttgga
     1321 aatcgaaggc cttgatgagc cgaccgttga agcactgcgc gagcgtgcta aaaatgcact
     1381 ggccaccatt gcacaggccc aggaagaaag cctcggtgat aacaaaccgg ctgacgatct
     1441 gctgaacctt gaaggggtag atcgtgattt ggcattcaaa ctggccgccc gtggcgtttg
     1501 tacgctggaa gatctcgccg aacagggcat tgatgatctg gctgatatcg aagggttgac
     1561 cgacgaaaaa gccggagcac tgattatggc tgcccgtaat atttgctggt tcggtgacga
     1621 agcgactaga tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     1681 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     1741 acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc
     1801 ggctcgtata atgtgtggat tttgagttag gatccggcga gattttcagg agctaaggaa
     1861 gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt
     1921 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     1981 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     2041 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa
     2101 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     2161 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     2221 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     2281 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     2341 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg
     2401 caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc
     2461 ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg
     2521 gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct
     2581 gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt
     2641 gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg
     2701 catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg
     2761 tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt
     2821 tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg
     2881 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
     2941 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
     3001 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
     3061 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
     3121 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag
     3181 gctccgttat acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta
     3241 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     3301 ttttacgttt ctcgttcagc tttcttgtac aaagtggttg attcgagcac caccaccacc
     3361 accactaatg ttaattaagt tgggcgttcc taggctgata aaacagaatt tgcctggcgg
     3421 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
     3481 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac
     3541 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     3601 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
     3661 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc
     3721 tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca
     3781 aatatgtatc cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     3841 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
     3901 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3961 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4021 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4081 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4141 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4201 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4261 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4321 caaaatatta acgtttacaa tttctggcgg cacgatggca tgagattatc aaaaaggatc
     4381 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4441 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4501 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4561 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4621 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4681 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4741 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4801 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4861 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4921 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4981 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5041 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5101 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5161 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5221 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5281 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatcatga
     5341 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5401 aaataaacaa ataggtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5461 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5521 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5581 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5641 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5701 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5761 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5821 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5881 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5941 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6001 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6061 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6121 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6181 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6241 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     6301 gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     6361 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     6421 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     6481 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     6541 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
     6601 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
     6661 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
     6721 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
     6781 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
     6841 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca
     6901 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg
     6961 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt
     7021 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt
     7081 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac
     7141 gacaggagca cgatcatgct agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     7201 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
     7261 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7321 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct
     7381 tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg
     7441 cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa
     7501 cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc
     7561 accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt
     7621 ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa
     7681 accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt
     7741 gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc
     7801 taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc
     7861 gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa
     7921 cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata
     7981 gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc
     8041 ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg
     8101 agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac
     8161 gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt
     8221 cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg
     8281 gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa
     8341 cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat
     8401 accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg
     8461 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
     8521 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     8581 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     8641 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     8701 tgcgtccggc gtagaggatc gaga
//
