LOCUS       sequence-69690-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible mRFP1-tagged 
            constitutively active MEK5 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Jensen KJ, Garmaroudi FS, Zhang J, Lin J, Boroomand S, Zhang M, Luo 
            Z, Yang D, Luo H, McManus BM, Janes KA
  TITLE     An ERK-p38 subnetwork coordinates host cell apoptosis and necrosis 
            during coxsackievirus B3 infection.
  JOURNAL   Cell Host Microbe. 2013 Jan 16;13(1):67-76. doi: 
            10.1016/j.chom.2012.11.009. Epub 2013 Jan 16.
  PUBMED    23332156
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2012.11.009"; journalName: "Cell Host Microbe"; 
            date: "2013-01-16- 16"; volume: "13"; issue: "1"; pages: "67-76"
COMMENT     Sequence Label: pSLIK MEK5-DD-mRFP1 neo
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    91..115
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        147..461
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    155..173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    226..244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    262..280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    298..316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    333..351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    369..387
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     434..458
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
       61 tccagtttgg ttaattctag acagttatca acaagtttgt acaaaaaagc aggctttaaa
      121 ggaaccaatt cagtcgactg gatcccgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttac tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
      301 ctatcagtga tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc
      361 gagtttactc cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg
      421 cctatataag cagagctcgt ttagtgaacc gtcagatcgc cactagtatg ctgtggctag
      481 cccttggccc ctttcctgcc atggagaacc aggtgctggt aattcgcatc aagatcccaa
      541 atagtggcgc ggtggactgg acagtgcact ccgggccgca gttactcttc agggatgtgc
      601 tggatgtgat aggccaggtt ctgcctgaag caacaactac agcatttgaa tatgaagatg
      661 aagatggtga tcgaattaca gtgagaagtg atgaggaaat gaaggcaatg ctgtcatatt
      721 attattccac agtaatggaa cagcaagtaa atggacagtt aatagagcct ctgcagatat
      781 ttccaagagc ctgcaagcct cctggggaac ggaacataca tggcctgaag gtgaatactc
      841 gggccggacc ctctcaacac ag
//
